Department of Pediatrics and Adolescent Medicine, University of Erlangen-Nuremberg, Erlangen, Germany.
Placenta. 2010 Mar;31(3):178-85. doi: 10.1016/j.placenta.2009.12.002. Epub 2010 Jan 4.
The human placenta as part of the feto-placental unit may influence fetal endocrine systems and may therefore represent a very important link between intrauterine growth restriction (IUGR) and metabolic disorders in later life. We aimed to analyze the effect of sample origin on gene expression of placental factors potentially involved in fetal programming in IUGR versus appropriate for gestational age growth (AGA) to standardize sample collection procedure for a multicenter approach.
Placental gene expression of insulin-like growth factor-binding protein (IGFBP)-1, prolactin, corticotropin releasing hormone (CRH) and leptin was measured and compared between proximal, intermediate and peripheral region of the placenta in 22 IUGR (proven by anomalous placental Doppler velocimetry) and 19 AGA neonates.
Whereas no difference in gene expression was seen in the proximal portion, in the intermediate placental region mRNA expression of IGFBP-1 (p = 0.01), prolactin (p = 0.04), CRH (p = 0.01) and leptin (p = 0.04) was increased in IUGR samples compared to controls. At the placental periphery, gene expression of these placental transcripts showed a higher expression level in IUGR placentas without statistical significance, except for leptin (p = 0.03).
Placental sampling site seems to be relevant for detecting differences in gene expression between IUGR and AGA neonates.
胎盘作为胎-胎盘单位的一部分，可能会影响胎儿的内分泌系统，因此它可能是宫内发育迟缓（IUGR）与儿童后期代谢紊乱之间的一个非常重要的联系环节。我们旨在分析胎盘因子的基因表达样本来源对 IUGR 与适当胎龄生长（AGA）之间的影响，以标准化多中心研究的样本采集程序。
在 22 例 IUGR（通过异常胎盘多普勒血流速度证明）和 19 例 AGA 新生儿中，测量并比较了胰岛素样生长因子结合蛋白（IGFBP）-1、催乳素、促肾上腺皮质激素释放激素（CRH）和瘦素在胎盘近端、中间和外周区域的基因表达。
虽然在胎盘近端部分没有观察到基因表达的差异，但在胎盘中间区域，IUGR 样本中 IGFBP-1（p=0.01）、催乳素（p=0.04）、CRH（p=0.01）和瘦素（p=0.04）的 mRNA 表达增加。在胎盘边缘，这些胎盘转录物的基因表达水平在 IUGR 胎盘中更高，但没有统计学意义，除了瘦素（p=0.03）。
胎盘采样部位似乎与检测 IUGR 和 AGA 新生儿之间基因表达的差异有关。
