Queen's Medical Centre, Nottingham, UK.
Cytokine. 2010 Apr;50(1):19-23. doi: 10.1016/j.cyto.2009.12.003. Epub 2010 Jan 4.
Th17 cells are thought to contribute to the immunopathology of allergic and autoimmune conditions. Their role in multiple sclerosis (MS) pathology remains to be fully elucidated.
To assess peripheral blood Th17 responses in patients with MS compared to controls.
We isolated peripheral blood mononuclear cells from 41 MS patients and 23 healthy controls, which were then stimulated using phorbol ester and ionomycin, labelled for CD3, CD8, CD154, IL-17 and IFN-gamma and analysed using flow cytometry.
Minimal IL-17 was detectable in unstimulated cells. Following stimulation with phorbol ester and ionomycin, PBMCs taken from MS patients in relapse developed a more inflammatory profile than those taken from controls or non-relapse patients, with greater expression of CD154, IL-17 and dual expression of IL-17/IFN-gamma.
We suggest a greater tendency to Th17 and Th1/Th17 response to non-specific stimulation in MS patients in relapse compared to controls and non-relapse patients.
Th17 细胞被认为有助于过敏和自身免疫性疾病的免疫病理学。它们在多发性硬化症（MS）发病机制中的作用仍有待充分阐明。
评估 MS 患者与对照组相比外周血 Th17 反应。
我们从 41 名 MS 患者和 23 名健康对照者中分离外周血单核细胞，然后使用佛波醇酯和离子霉素刺激，用 CD3、CD8、CD154、IL-17 和 IFN-γ标记，并通过流式细胞术进行分析。
未刺激细胞中可检测到最小量的 IL-17。在用佛波醇酯和离子霉素刺激后，来自复发 MS 患者的 PBMC 比来自对照者或非复发患者的 PBMC 表现出更具炎症特征，表现为 CD154、IL-17 和 IL-17/IFN-γ双重表达增加。
与对照者和非复发患者相比，我们发现在复发 MS 患者中，非特异性刺激下 Th17 和 Th1/Th17 反应有更大的倾向。
