Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, 10 Xitoutiao, You An Men, Beijing 100069, PR China.
Exp Parasitol. 2010 Apr;124(4):403-8. doi: 10.1016/j.exppara.2009.12.010. Epub 2010 Jan 4.
Our previous studies showed that immunization with recombinant paramyosin from Trichinella spiralis (rTs-Pmy) formulated with Freund's adjuvant significantly reduced larval burden in mice after T. spiralis larval challenge. Since Freund's adjuvant is toxic and not a suitable adjuvant for clinical vaccine trials, we evaluated the ability of the adjuvants Montanide ISA206 and ISA720 to stimulate immune responses during rTs-Pmy immunization and to enhance protective immunity. The results revealed that immunization of BALB/c mice with rTs-Pmy formulated with either ISA206 or ISA720 triggered Th1 and Th2 immune responses similar to those produced by the conventional Freund's adjuvant formulation and also provided a similar level of protection against T. spiralis larval challenge. This indicates that the recombinant Ts-Pmy formulated with Montanide ISA206 or ISA720 may be an effective and safety vaccine strategy for trichinellosis.
我们之前的研究表明，用弗氏佐剂配制的旋毛虫重组肌球蛋白（rTs-Pmy）免疫可以显著降低旋毛虫幼虫感染后小鼠幼虫的负荷。由于弗氏佐剂有毒，不适合临床疫苗试验，因此我们评估了 Montanide ISA206 和 ISA720 佐剂在 rTs-Pmy 免疫期间刺激免疫反应和增强保护免疫的能力。结果表明，用 ISA206 或 ISA720 配制的 rTs-Pmy 免疫 BALB/c 小鼠会引发 Th1 和 Th2 免疫反应，类似于常规弗氏佐剂配方产生的反应，并且对旋毛虫幼虫感染也提供了类似水平的保护。这表明，用 Montanide ISA206 或 ISA720 配制的重组 Ts-Pmy 可能是一种有效的、安全的旋毛虫病疫苗策略。
