Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF.
Vet Rec. 2010 Jan 2;166(1):10-4. doi: 10.1136/vr.b5583.
The progress and pathogenesis of foot-and-mouth disease virus (FMDV) was studied in infected pigs by observing the development of clinical signs in two separate experiments. Viral loads were determined by real-time quantitative RT-PCR in the liver, spleen, cervical lymph node, mandibular lymph node, retropharyngeal lymph node, soft palate, pharynx, tonsil, tongue and skin (coronary band area). Tissue samples were collected from both inoculated and contact-infected pigs at several time points during infection, and blood samples were collected to assess viraemia and its relationship to tissue viral load. Virus first appeared in the lymph nodes, followed by viraemia and then clinical signs. The results suggested that FMDV accumulated in lymphoid tissue up to six hours after infection, in the tissues drained by the mandibular lymph node and tonsil and then disseminated throughout the body where epithelial cells were the favoured sites of replication.
本研究通过观察两批试验感染猪的临床症状发展，研究了口蹄疫病毒（FMDV）的进展和发病机制。通过实时定量 RT-PCR 法检测了猪的肝脏、脾脏、颈淋巴结、下颌淋巴结、咽后淋巴结、软腭、咽、扁桃体、舌和皮肤（冠状带区）中的病毒载量。在感染过程中，分别从接种感染和接触感染的猪中采集多个时间点的组织样本和血液样本，以评估病毒血症及其与组织病毒载量的关系。结果表明，病毒首先出现在淋巴结中，随后出现病毒血症，最后出现临床症状。研究结果表明，FMDV 在感染后 6 小时内就在淋巴组织中积累，在颌下淋巴结和扁桃体引流的组织中，并随后在全身扩散，其中上皮细胞是病毒复制的首选部位。
