Riley J C, Behrman H R
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510-8063.
Endocrinology. 1991 Apr;128(4):1749-53. doi: 10.1210/endo-128-4-1749.
The hypothesis that hydrogen peroxide generation occurs in the corpora lutea of superovulated rats during luteolysis was tested using a peroxide-dependent inhibitor of catalase, 3-amino-1,2,4-triazole (AT). Luteal regression was induced during midpseudopregnancy by injection of 500 micrograms prostaglandin F2 alpha (PGF2 alpha) 1 h before administration of AT (0.1 g/kg, ip) and was confirmed by progesterone analysis of peripheral blood serum. Within groups of both PGF2 alpha-treated and untreated control rats, other rats also received ethanol (0.2 g/kg, ip), which prevents hydrogen peroxide-mediated inhibition of catalase by AT. Diluted homogenates of ovaries removed 1 h after AT administration were assayed for catalase activity by measuring the decrease in absorbance at 240 nm for 30 sec after the addition of hydrogen peroxide (10 mM). Ethanol-sensitive catalase inhibition by AT was significantly higher (47.9 +/- 3.38%) in samples from PGF2 alpha-treated groups than in controls (23.1 +/- 4.82%; P less than 0.01; n = 9). Similar increases in catalase inhibition by AT were found in luteal tissue of rats treated with PGF2 alpha 24 h earlier and in rats in which luteolysis was allowed to occur spontaneously in late pseudopregnancy. Hemoglobin an AT assays revealed that the changes in catalase activity were not the result of altered blood contamination or AT concentration in the luteal homogenates. Since catalase inhibition by AT is only seen in the presence of hydrogen peroxide, these results support the conclusion that an early and sustained component of corpus luteum regression is the generation of hydrogen peroxide in luteal tissue.
使用过氧化氢酶的过氧化物依赖性抑制剂3-氨基-1,2,4-三唑（AT），对超排卵大鼠黄体溶解期间黄体中过氧化氢生成的假说进行了验证。在假孕中期，于给予AT（0.1 g/kg，腹腔注射）前1小时注射500微克前列腺素F2α（PGF2α）诱导黄体退化，并通过外周血血清孕酮分析加以证实。在接受PGF2α处理和未处理的对照组大鼠中，其他大鼠还接受了乙醇（0.2 g/kg，腹腔注射），乙醇可防止过氧化氢介导的AT对过氧化氢酶的抑制作用。在给予AT 1小时后取出卵巢，制备稀释匀浆，通过加入过氧化氢（10 mM）后测量240 nm处吸光度在30秒内的下降来测定过氧化氢酶活性。来自PGF2α处理组的样本中，AT对乙醇敏感的过氧化氢酶抑制作用显著高于对照组（47.9±3.38%比23.1±4.82%；P<0.01；n = 9）。在用PGF2α处理24小时的大鼠黄体组织以及在假孕后期自然发生黄体溶解的大鼠中，也发现了AT对过氧化氢酶抑制作用的类似增加。血红蛋白和AT检测表明，过氧化氢酶活性的变化不是黄体匀浆中血液污染改变或AT浓度改变的结果。由于只有在过氧化氢存在的情况下才会出现AT对过氧化氢酶的抑制作用，这些结果支持以下结论：黄体退化的早期持续性成分是黄体组织中过氧化氢的生成。
