Paraszkiewicz Katarzyna, Bernat Przemysław, Naliwajski Marcin, Długoński Jerzy
Department of Industrial Microbiology and Biotechnology, University of Łódź, Banacha 12/16, 90-237, Lodz, Poland.
Arch Microbiol. 2010 Feb;192(2):135-41. doi: 10.1007/s00203-009-0542-3. Epub 2010 Jan 3.
The effect of Ni(2+) on fungal growth, cellular fatty acid profile and lipid peroxidation was studied (with an emphasis on the kinetics of these processes) in the strain of filamentous fungus Curvularia lunata. In the cultures supplemented with 0.2 and 0.6 mM Ni(2+) the lag phase was extended and the specific growth rate decreased, however, the maximum yield of biomass at the stationary phase reached, respectively, 97 and 27% of the control. The treatment with Ni(2+) changed the proportion of 18 C atom fatty acids, with the most significant decrease in the content of linoleic acid (18:2) followed by a rise in the degree of fatty acid saturation. In the mycelia exposed to Ni(2+) the levels of TBARS (lipid peroxidation products) increased and ranged between 156 and 823% over the control. The presented data reveal that the oxidative stress resulting, among others, in membrane lipid peroxidation is involved in the mechanisms of the nickel toxicity towards C. lunata and suggest that this fungus exhibits an ability to cope, to some extent, with the increased level of lipid peroxides.
研究了Ni(2+)对丝状真菌新月弯孢菌菌株的真菌生长、细胞脂肪酸谱和脂质过氧化的影响（重点关注这些过程的动力学）。在添加了0.2和0.6 mM Ni(2+)的培养物中，延迟期延长，比生长速率降低，然而，稳定期的生物量最大产量分别达到对照的97%和27%。Ni(2+)处理改变了18碳脂肪酸的比例，亚油酸（18:2）含量下降最为显著，随后脂肪酸饱和度增加。在暴露于Ni(2+)的菌丝体中，TBARS（脂质过氧化产物）水平升高，比对照高出156%至823%。所呈现的数据表明，氧化应激（尤其是导致膜脂质过氧化）参与了镍对新月弯孢菌的毒性机制，并表明这种真菌在一定程度上具有应对脂质过氧化物水平升高的能力。
