面向大众的磷酸化蛋白质组学。
Phosphoproteomics for the masses.
机构信息
Department of Chemistry,University of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
ACS Chem Biol. 2010 Jan 15;5(1):105-19. doi: 10.1021/cb900277e.
Abstract
Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of biological organisms. Technical advancements over the last several years in mass spectrometry now allow for the large-scale identification and quantitation of in vivo phosphorylation at unprecedented levels. These developments have occurred in the areas of sample preparation, instrumentation, quantitative methodology, and informatics so that today, 10 000-20 000 phosphorylation sites can be identified and quantified within a few weeks. With the rapid development and widespread availability of such data, its translation into biological insight and knowledge is a current obstacle. Here we present an overview of how this technology came to be and is currently applied, as well as future challenges for the field.
摘要
蛋白质磷酸化是生物体内细胞信号转导的主要机制。近年来，质谱技术的进步使得在体内大规模鉴定和定量磷酸化成为可能，达到了前所未有的水平。这些进展发生在样品制备、仪器、定量方法和信息学等领域，以至于现在可以在短短几周内鉴定和定量 10000-20000 个磷酸化位点。随着这些数据的快速发展和广泛应用，将其转化为生物学见解和知识是当前的一个障碍。本文综述了该技术的发展历程和现状，以及该领域未来面临的挑战。
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