将神经元烟碱型乙酰胆碱受体的多个亚基定位到人类的15号染色体和小鼠的9号染色体上。

Mapping of multiple subunits of the neuronal nicotinic acetylcholine receptor to chromosome 15 in man and chromosome 9 in mouse.

作者信息

Eng C M, Kozak C A, Beaudet A L, Zoghbi H Y

机构信息

Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.

出版信息

Genomics. 1991 Feb;9(2):278-82. doi: 10.1016/0888-7543(91)90253-b.

DOI:10.1016/0888-7543(91)90253-b

PMID:2004777

Abstract

The alpha 3, alpha 5, and beta 4 genes (human gene symbols CHRNA3, CHRNA5, and CHRNB4 respectively; mouse gene symbols Acra-3, Acra-5, and Acrb-4, respectively) are members of the nicotinic acetylcholine receptor gene family and are clustered within a 68-kb segment of the rat genome (Boulter et al., 1990, J. Biol. Chem. 265:4472). By somatic cell hybrid analysis, three cDNAs corresponding to these genes were used to map the homologous loci to human chromosome 15 and to mouse chromosome 9. Linkage analysis using CEPH pedigrees showed that the CHRNA5 gene was closely linked to the following chromosome 15 loci: D15S46, D15S52, D15S28, D15S34, and D15S35. Using interspecies crosses in mice, the Acra-5 gene was found closely linked to the Mpi-1 locus. The mapping of these members of a neurotransmitter receptor gene family may facilitate the identification of relationships between the neurotransmitter receptors and murine or human phenotypes.

摘要

α3、α5和β4基因（人类基因符号分别为CHRNA3、CHRNA5和CHRNB4；小鼠基因符号分别为Acra-3、Acra-5和Acrb-4）是烟碱型乙酰胆碱受体基因家族的成员，在大鼠基因组的一个68 kb片段内成簇排列（Boulter等人，1990年，《生物化学杂志》265:4472）。通过体细胞杂交分析，与这些基因对应的三个cDNA被用于将同源基因座定位到人类15号染色体和小鼠9号染色体上。使用CEPH家系进行的连锁分析表明，CHRNA5基因与以下15号染色体基因座紧密连锁：D15S46、D15S52、D15S28、D15S34和D15S35。在小鼠中进行种间杂交时，发现Acra-5基因与Mpi-1基因座紧密连锁。对这个神经递质受体基因家族成员的定位可能有助于确定神经递质受体与小鼠或人类表型之间的关系。