序批式反应器培养自养硝化颗粒的运行。

Operation of a sequencing batch reactor for cultivating autotrophic nitrifying granules.

机构信息

Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

出版信息

Bioresour Technol. 2010 May;101(9):2960-4. doi: 10.1016/j.biortech.2009.11.099. Epub 2010 Jan 4.

DOI:10.1016/j.biortech.2009.11.099

PMID:20047829

Abstract

The granulation of nitrifying sludge in a sequencing batch reactor (SBR) fed with NH(4)(+)-N-laden inorganic wastewater was investigated. After 120-day operation spherical and elliptical granules with an average diameter of 0.32 mm were observed. The hydrophobicity surface, settling velocity and specific gravity of the matured granules increased with the processing of sludge granulation. Spatial distribution of bacterial species within the autotrophic granules was analyzed with fluorescence in situ hybridization. Both ammonia- and nitrite-oxidizing bacteria were observed in the granular sludge. The Michaelis-Menten equation was used to describe their NH(4)(+)-N utilization rate, and the kinetic coefficients were calculated to be v(m) = 18.0 mg/g-VSS/h and K(m) = 36.7 mg/l. Taking into account the NH(4)(+)-N utilization rate and removal efficiency together, an NH(4)(+)-N concentration range of 100-250 mg/l was found to be favourable for the operation of the SBR to cultivate nitrifying granules.

摘要

采用 NH(4)(+)-N 负荷无机废水对序批式反应器（SBR）中的硝化污泥进行颗粒化研究。经过 120 天的运行，观察到直径为 0.32mm 的球形和椭圆形颗粒。成熟颗粒的疏水性表面、沉淀速度和比重随污泥颗粒化过程而增加。利用荧光原位杂交技术分析了自养颗粒内细菌种类的空间分布。在颗粒污泥中均观察到氨氧化菌和亚硝酸盐氧化菌。采用米氏方程描述其 NH(4)(+)-N 利用率，并计算出动力学系数分别为 v(m) = 18.0mg/g-VSS/h 和 K(m) = 36.7mg/l。综合考虑 NH(4)(+)-N 利用率和去除效率，发现 100-250mg/l 的 NH(4)(+)-N 浓度范围有利于 SBR 运行以培养硝化颗粒。