Department of Pharmacy and Pharmacology, University of Bath, Bath, UK.
Skin Pharmacol Physiol. 2010;23(3):117-23. doi: 10.1159/000270381. Epub 2009 Dec 23.
The objective of this preliminary investigation was to examine the disposition of charged nanoparticles on and within the skin following their topical application and to explore whether the formulations have potential utility for the local delivery of an associated 'active' substance. Three nanoparticles (approx. 100 nm in diameter) were investigated: cationic amino-functionalized polystyrene, an anionic carboxyl-functionalized polystyrene and anionic poly-(L-lactide), into each of which the fluorophore N-(2,6-diisopropylphenyl)perylene-3,4-dicarboximine (PMI) was incorporated. Formulations were applied to excised porcine skin in vitro for 6 h. After cleaning the skin surface following treatment, the skin was either examined by laser scanning confocal microscopy or subjected to repeated tape-stripping and subsequent analysis of the removed stratum corneum (SC) for the presence of PMI. The cationic nanoparticles showed clear affinity for the negatively charged skin surface (in contrast to the anionic carriers) and delivered a significantly greater amount of the model 'active' agent (PMI) into the SC.
本初步研究旨在考察经皮给药后带电荷纳米颗粒在皮肤表面和皮肤内的分布情况，并探索这些制剂是否有潜力用于局部递送相关的“活性”物质。研究了三种纳米颗粒（直径约 100nm）：阳离子氨基功能化聚苯乙烯、阴离子羧基功能化聚苯乙烯和阴离子聚（L-丙交酯），其中每种纳米颗粒都掺入了荧光团 N-（2,6-二异丙基苯基）苝-3,4-二羧酸亚胺（PMI）。将制剂以 6 小时的时间间隔应用于离体猪皮。治疗后，在清洁皮肤表面后，通过激光扫描共聚焦显微镜检查皮肤，或进行多次胶带剥离，随后分析去除的角质层（SC）中是否存在 PMI。阳离子纳米颗粒对带负电荷的皮肤表面具有明显的亲和力（与阴离子载体相反），并将更多的模型“活性”试剂（PMI）递送至 SC 中。
