School of Life Science, Huzhou University, 313000, Huzhou, People's Republic of China.
Ecotoxicology. 2010 Apr;19(4):796-803. doi: 10.1007/s10646-009-0456-5. Epub 2010 Jan 6.
The bioaccumulation and harmful effects of microcystins (MCs) and the activity of peroxidase (POD) and superoxide dismutase (SOD) were examined in the apple (Malus pumila) exposed in vitro with the crude extract of toxic cyanobacterial blooms from Dianchi Lake in southwestern China. The results showed that the growth and proliferation of M. pumila shoots in vitro decreased markedly after exposure to microcystins above 0.3 microg/ml. Recovered microcystins determined by enzyme-linked immunosorbent assay (ELISA) in M. pumila shoot cultures increased with exposure time and concentration. After 14 days exposure to the concentration of 3 microg/ml microcystins, M. pumila shoot cultures accumulated microcystins up to a concentration of 510.23 +/- 141.10 ng MC-LR equiv/g FW (fresh weight), equivalent to an accumulation rate of 36.45 ng/g day. POD activity was significantly increased after 7 days exposure to 3 microg/ml microcystins. After 14 days of exposure, microcystins caused POD to increase significantly at the concentration of 0.3 and 3 microg/ml. The activity of SOD was not affected by microcystins at concentrations up to 3 microg/ml on 7 days. After 14 days exposure to microcystins, SOD activity increased significantly at the concentration of 0.3 and 3 microg/ml in M. pumila shoot cultures.
采用中国西南滇池有毒蓝藻水华粗提物对离体苹果（Malus pumila）进行处理，检测了微囊藻毒素（MCs）的生物累积及其对过氧化物酶（POD）和超氧化物歧化酶（SOD）活性的有害影响。结果表明，当暴露于 0.3μg/ml 以上的微囊藻毒素时，离体苹果茎的生长和增殖明显下降。通过酶联免疫吸附测定（ELISA）在苹果茎培养物中测定的回收微囊藻毒素随暴露时间和浓度而增加。在 3μg/ml 微囊藻毒素浓度下暴露 14 天后，苹果茎培养物积累的微囊藻毒素达到 510.23±141.10ng MC-LR equiv/gFW（鲜重），相当于 36.45ng/g 天的积累率。暴露于 3μg/ml 微囊藻毒素 7 天后，POD 活性显著增加。暴露 14 天后，0.3 和 3μg/ml 浓度的微囊藻毒素导致 POD 显著增加。在 3μg/ml 浓度下，SOD 活性在 7 天内不受微囊藻毒素的影响。暴露于微囊藻毒素 14 天后，0.3 和 3μg/ml 浓度的 SOD 活性在苹果茎培养物中显著增加。
