Huang Peng, Lin Li Min, Wu Xiao Ying, Tang Qiu Ling, Feng Xue Yong, Lin Guang Yu, Lin Xiaobo, Wang Hong Wu, Huang Tian Hua, Ma Lian
Department of Pediatrics, Second Affiliated Hospital of Shantou University Medical College (SUMC), Shantou, PR China.
J Cell Biochem. 2010 Mar 1;109(4):747-54. doi: 10.1002/jcb.22453.
Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed "tadpole-like" cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUF5), Ckit, CD49(f) (alpha6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage.
最近的研究表明，间充质干细胞在适当条件下可分化为生殖细胞。我们试图确定人脐带华通氏胶源性间充质干细胞（HUMSCs）是否能在体外形成生殖细胞。将HUMSCs置于全反式维甲酸、睾酮以及由新生雄性小鼠睾丸制备的睾丸细胞条件培养基中，诱导其分化为生殖细胞。用不同试剂诱导后，HUMSCs形成了“蝌蚪样”细胞，并呈现出生殖细胞特异性标志物Oct4（POUF5）、Ckit、CD49(f)（α6）、Stella（DDPA3）和Vasa（DDX4）的mRNA及蛋白表达。我们的结果可能为涉及HUMSCs的生殖治疗提供一条新途径，并为研究调控哺乳动物生殖系发育的分子机制提供一个新的体外模型。
