Department of Animal Biochemistry and Physiology, University of Life Sciences in Lublin, 20-033 Lublin, ul. Akademicka 12, Poland.
J Dairy Res. 2010 Feb;77(1):117-22. doi: 10.1017/S0022029909990458. Epub 2010 Jan 7.
The aim of the study was to evaluate the profile of antioxidant parameters in ewes' colostrum and milk in relation to breed during 5 d post partum. Total antioxidant capacity (TAC) was analysed and the activity of the enzymic antioxidants, glutathione peroxidase (GSH-Px) and glutathione transferase (GSH-Tr), as well as the concentration of the non-enzymic antioxidants, vitamin C, vitamin A and beta-carotene, were measured. Samples were collected from healthy animals belonging to two ewe breeds: Berrichon du Cher (n=15) and Uhrusk (n=15) kept in the Podlasie Province (Poland). Colostrum was sampled directly after parturition, as well as after 12, 24 and 48 h later and milk was sampled 5 d after parturition. Colostrum and milk for the evaluation of all parameters except for vitamin A and beta-carotene were centrifuged, and the supernatant was used for further analysis. Spectrophotometric methods were used for biochemical measurements. The results showed dynamic changes of antioxidative parameters within the time period examined. TAC values and GSH-Px activity increased significantly during the experiment. GSH-Tr activity showed a similar tendency in Uhrusk ewes but an opposite relationship in Berrichon du Cher. Concentrations of examined vitamins followed the increasing trends noticed in the activities of antioxidative enzymes. Moreover, differences between breeds in the evaluated parameters were detected; these differences were not unequivocal however. The results are also a source of not previously published physiological antioxidant profile in colostrum and milk of ewes over the post-partum period.
本研究旨在评估产后 5 天内不同品种绵羊乳及初乳中抗氧化参数的特征。分析总抗氧化能力（TAC），并检测抗氧化酶（谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽转移酶（GSH-Tr））的活性以及非酶抗氧化剂（维生素 C、维生素 A 和β-胡萝卜素）的浓度。从属于两个绵羊品种（Berrichon du Cher 和 Uhrusk）的健康动物中采集样本，这些动物饲养在波兰波德拉谢省。产后直接采集初乳，之后分别在 12、24 和 48 小时后采集初乳，产后 5 天采集乳样。用于评估所有参数（除维生素 A 和β-胡萝卜素）的乳样均离心，上清液用于进一步分析。使用分光光度法进行生化测量。结果显示，在研究期间抗氧化参数呈现动态变化。TAC 值和 GSH-Px 活性在实验过程中显著增加。GSH-Tr 活性在 Uhrusk 绵羊中呈现出相似的趋势，但在 Berrichon du Cher 中则呈相反的关系。所检测维生素的浓度与抗氧化酶活性的增加趋势一致。此外，还检测到了品种间评估参数的差异；但这些差异并不明确。这些结果还提供了之前未发表的关于绵羊产后期间乳及初乳中抗氧化生理特征的信息。
