Department of Parasitology, Mycology and Tropical Medicine, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
J Antimicrob Chemother. 2010 Mar;65(3):438-41. doi: 10.1093/jac/dkp467. Epub 2010 Jan 6.
The frequency of dhfr and dhps point mutations was assessed in Plasmodium falciparum isolates from pregnant women in Libreville.
PCR-restriction fragment length polymorphism of polymorphic codons of the dhfr gene (51, 59 and 108) and the dhps gene (436, 437 and 540) was performed in matched peripheral and placental blood samples.
The proportion of multiple mutations was high (98%), and was not different between women with and without a history of intermittent preventive treatment with sulfadoxine/pyrimethamine (IPTp/SP). The prevalence of triple dhfr mutation was 80%, and that of quadruple and quintuple mutations was 53% and 22%, respectively. The Glu540 mutation was present in two isolates. The concordance of resistant alleles in matched peripheral and placental isolates was >90% for both genes.
These findings underline the need for a regular assessment of the relationship between the presence of resistant isolates and in vitro/in vivo IPTp/SP efficacy, and evaluation of an alternative drug.
评估利伯维尔孕妇体内疟原虫 falciparum 分离株 dhfr 和 dhps 点突变的频率。
对 dhfr 基因（51、59 和 108）和 dhps 基因（436、437 和 540）的多态性密码子进行 PCR-限制性片段长度多态性分析，分别在配对的外周血和胎盘血样本中进行。
多重突变的比例很高（98%），且在有或无磺胺多辛/乙胺嘧啶间歇性预防治疗（IPTp/SP）史的妇女之间无差异。三重 dhfr 突变的流行率为 80%，四重和五重突变的流行率分别为 53%和 22%。有两个分离株存在 Glu540 突变。两个基因在配对的外周和胎盘分离株中的耐药等位基因的一致性>90%。
这些发现强调了需要定期评估耐药分离株的存在与体外/体内 IPTp/SP 疗效之间的关系，并评估替代药物。
