Lakkakorpi J T, Rajaniemi H J
Biocenter University of Oulu, Finland.
J Histochem Cytochem. 1991 Apr;39(4):397-400. doi: 10.1177/39.4.2005369.
We used confocal scanning microscopy to study the semi-quantitative distribution of luteinizing hormone/chorionic gonadotropin (LH/CG) receptors on rat luteal cells at both the two- and the three-dimensional level. The receptors were visualized in 6-microns sections of pseudopregnant rat ovaries using polyclonal rabbit antiserum to hCG-affinity-purified LH/CG receptor in conjunction with rhodamine-conjugated anti-rabbit immunoglobulins. Twenty to 30 optical sections were taken at different focal planes from representative luteal cells with a confocal laser scanning microscope and then processed digitally to two- and three-dimensional pseudocolored images. Distinct differences in fluorescence intensity could be demonstrated at both the two- and the three-dimensional level on the luteal cell surfaces, suggesting an uneven distribution of the LH/CG receptors on the cell membranes. This probably results in the compartmentalization and polarization of luteal cell function.
我们使用共聚焦扫描显微镜在二维和三维水平上研究促黄体生成素/绒毛膜促性腺激素（LH/CG）受体在大鼠黄体细胞上的半定量分布。使用针对人绒毛膜促性腺激素亲和纯化的LH/CG受体的多克隆兔抗血清，结合罗丹明偶联的抗兔免疫球蛋白，在假孕大鼠卵巢的6微米切片中观察受体。用共聚焦激光扫描显微镜在不同焦平面从代表性黄体细胞采集20至30个光学切片，然后进行数字处理以生成二维和三维伪彩色图像。在黄体细胞表面的二维和三维水平上都能显示出荧光强度的明显差异，这表明LH/CG受体在细胞膜上分布不均。这可能导致黄体细胞功能的区室化和极化。
