Department of Microbial Biotechnology, Institute of Biochemistry and Biotechnology, Faculty of Life Sciences, Martin-Luther-University Halle-Wittenberg, Germany.
J Gen Virol. 2010 May;91(Pt 5):1213-7. doi: 10.1099/vir.0.016998-0. Epub 2010 Jan 6.
Here we report on a segment in the genomic 3' non-translated region (3'NTR) of bovine viral diarrhea virus (BVDV) that is accessible for the insertion of foreign sequence elements such as the 5'NTR of hepatitis C virus. Recombinant viruses exhibited replication kinetics similar to those of the parental strain, and characterization of RNA species after several passages revealed that foreign inserts had the same genetic stability as the BVDV 3'NTR. The generation of such BVDV recombinants is relevant for several applications.
在这里，我们报告了牛病毒性腹泻病毒（BVDV）基因组 3'非翻译区（3'NTR）的一个片段，该片段可插入外来序列元件，如丙型肝炎病毒的 5'NTR。重组病毒表现出与亲本株相似的复制动力学，并且经过多次传代后 RNA 种类的特征表明，外源插入与 BVDV 3'NTR 具有相同的遗传稳定性。此类 BVDV 重组病毒的产生与多种应用相关。
