School of Life Sciences, Devi Ahilya University, Indore, 452 001, M.P, India.
J Bioenerg Biomembr. 2010 Feb;42(1):47-53. doi: 10.1007/s10863-009-9260-4. Epub 2010 Jan 7.
Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.
氯是氧释放复合体（OEC）发挥功能所必需的因素，对光系统 II 具有保护和激活作用。在这项研究中，我们主要通过电子顺磁共振（EPR）研究了菠菜的含氯充足、缺氯和去氯类囊体膜和富含光系统 II 的膜的性质。不同的去氯方法对光系统 II 结构和功能方面的影响的结果表明，用高 pH 值处理 PS II 膜进行去氯是一种相对苛刻的方法，会对 PS II 的供体侧造成严重且不可修复的损伤。在缺氯和去氯 PS II 膜中，PS II 的受体侧的损伤几乎完全得到恢复。
