芹菜素分子印迹聚合物的制备及光谱表征

[Preparation and spectral characterization of apigenin molecularly imprinted polymer].

作者信息

Li Li-Li, Chen Xiao-Ming

机构信息

College of Chemistry, Xiangtan University, Hunan 411105, China.

出版信息

Yao Xue Xue Bao. 2009 Aug;44(8):868-72.

PMID:20055154

Abstract

An apigenin molecularly imprinted polymer was prepared by bulk polymerization using apigenin as template, acrylamide (AA) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a crosslinking agent, acetonitrile and N, N-dimethylformamide as porogenic solvents, and 2, 2'-azobisisobutyronitrile (AIBN) as an initiator. The interaction between template and functional monomer was proved by ultraviolet visible (UV) spectrophotometry and based on the results, appropriate reaction solvent was selected and the synthesizing process was estimated. The molecularly imprinted polymer structure was analyzed by Fourier Transform Infrared (FT-IR) spectrophotometry. The molecularly imprinted polymer was investigated in equilibrium binding experiment to evaluate its adsorption property, the results showed that the adsorption of apigenin on molecularly imprinted polymer is higher than that on blank polymer in the studied concentration range (0.1-2.5 mmol x L(-1)). Scatchard analysis showed that two classes of binding sites existed in the apigenin imprinted polymers, with their KD and Qmax estimated to be 2.52 x 10(-4), 0.54 x 10(-3) mmol x L(-1) and 2.65, 18.89 micromol x g(-1), respectively. Molecularly imprinted polymer showed higher affinity than blank polymer.

摘要

以芹菜素为模板，丙烯酰胺（AA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，乙腈和N，N -二甲基甲酰胺为致孔剂，2,2'-偶氮二异丁腈（AIBN）为引发剂，通过本体聚合法制备了芹菜素分子印迹聚合物。通过紫外可见（UV）分光光度法证明了模板与功能单体之间的相互作用，并基于该结果选择了合适的反应溶剂并评估了合成过程。通过傅里叶变换红外（FT-IR）分光光度法分析了分子印迹聚合物的结构。在平衡结合实验中对分子印迹聚合物进行了研究以评估其吸附性能，结果表明在所研究的浓度范围（0.1 - 2.5 mmol·L⁻¹）内，芹菜素在分子印迹聚合物上的吸附高于在空白聚合物上的吸附。Scatchard分析表明，芹菜素印迹聚合物中存在两类结合位点，其KD和Qmax估计分别为2.52×10⁻⁴、0.54×10⁻³ mmol·L⁻¹和2.65、18.89 μmol·g⁻¹。分子印迹聚合物表现出比空白聚合物更高的亲和力。