Ge Jin, Zhao Liang, Chen Li-Ren, Shi Yan-Ping
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
J Chromatogr Sci. 2010 Jan;48(1):29-34. doi: 10.1093/chromsci/48.1.29.
Titania-coated silica spheres were prepared by a layer-by-layer self-assembly technique for use as a high-performance liquid chromatography packing. This packing has a high surface area of 202.1 m(2)/g, a large pore volume of 0.36 cm(3)/g, and a pore diameter of 7.0 nm. Furthermore, the packing particles exhibit narrow pore size distribution and good pore structure. The chromatographic behavior of the packing was studied under both normal and reversed-phase conditions. Low column pressures were observed. Aromatic isomeric compounds were well separated on the TiO(2)/SiO(2) column under normal phase conditions; pyridine and aniline derivatives were separated on the octadecyl-bonded TiO(2)/SiO(2) (ODT) column, respectively, under reversed-phase conditions, and highly symmetrical peaks were obtained for 15 basic compounds. The chemical stability of the stationary phase was tested with sodium phosphate solution (10 mmol/L, pH = 10) at 25 degrees C and potassium phosphate solution (50 mmol/L, pH = 10) at 50 degrees C.
通过层层自组装技术制备了二氧化钛包覆的硅胶球，用作高效液相色谱填料。该填料具有202.1 m²/g的高比表面积、0.36 cm³/g的大孔体积和7.0 nm的孔径。此外，填料颗粒呈现出窄的孔径分布和良好的孔结构。在正相和反相条件下研究了该填料的色谱行为。观察到较低的柱压。在正相条件下，芳香族异构体化合物在TiO₂/SiO₂柱上得到良好分离；在反相条件下，吡啶和苯胺衍生物分别在十八烷基键合的TiO₂/SiO₂（ODT）柱上分离，并且15种碱性化合物获得了高度对称的峰。在25℃下用磷酸钠溶液（10 mmol/L，pH = 10）和在50℃下用磷酸钾溶液（50 mmol/L，pH = 10）测试了固定相的化学稳定性。
