[用表达乙肝病毒截短C基因和前S1基因的卡介苗免疫BALB/c小鼠后的免疫反应]
[Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene].
作者信息
Yin Wen, Lü Xin, Lei Ying-feng, Yang Jing, Sun Meng-ning, Ma Yu, Lan Hai-yun, Wang Ai-qin
机构信息
Center Laboratory, Fourth Medical Military University, Xi'an 710032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jan;26(1):28-30.
AIM
To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.
METHODS
A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.
RESULTS
Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.
CONCLUSION
BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
目的
研究乙肝病毒截短核心蛋白和前S1蛋白在卡介苗中的表达情况，并探讨重组卡介苗刺激体液免疫和细胞免疫反应的效果。
方法
构建穿梭载体并将其转化至包含乙肝病毒截短核心基因和前S1基因的卡介苗中。采用SDS-PAGE和Western blot对重组卡介苗进行分析。将三组BALB/c小鼠分别用生理盐水、卡介苗、卡介苗-pDE22和卡介苗-pDE22-CS1进行免疫。然后评估抗体效价和CTL效应。
结果
与对照组相比，重组卡介苗可表达一条24 kD的新蛋白条带，其大小与CS1融合蛋白一致，Western blot分析显示其具有良好的抗原结合特性。重组卡介苗免疫组的抗体效价显著升高，CTL效应明显增强。
结论
卡介苗可作为携带乙肝病毒相关基因的活载体，有望开发出新型抗乙肝疫苗。
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