Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, 10555 W Flagler Street, Miami, FL 33174, USA.
Biosens Bioelectron. 2010 Mar 15;25(7):1829-33. doi: 10.1016/j.bios.2009.12.012. Epub 2009 Dec 22.
The possibility of employing graphene sheets as a potential candidate for the construction of biofuel cells is reported in this paper. Initially, graphene sheets were chemically synthesized and characterized by surface characterization techniques. Following this, graphene was employed to fabricate the anode and cathode in the biofuel cell. The anode of the biofuel cell consists of a gold electrode on which we co-immobilized graphene - glucose oxidase using silica sol-gel matrix. Voltammetric measurements were conducted to quantitatively evaluate the suitability of employing graphene sheets as an electrode dopant and its performance was compared with single walled carbon nanotubes (SWCNTs). The cathode of the biofuel cell was constructed in a similar method except that graphene was co-immobilized with bilirubin oxidase. Finally, two membraneless enzymatic biofuel cells, one using graphene sheets and the other using SWCNTs, were constructed and their performances were compared. Upon comparison, graphene based biofuel cell exhibited a maximum power density of about 24.3+/-4 microW (N=3), which is nearly two times greater than that of the SWCNTs biofuel cell, and the performance of the graphene biofuel cell lasted for 7 days.
本文报道了将石墨烯片作为构建生物燃料电池的潜在候选材料的可能性。首先，通过表面特征技术对石墨烯片进行了化学合成和特性描述。随后，将石墨烯用于生物燃料电池的阳极和阴极的制造。生物燃料电池的阳极由金电极组成，我们在其上共同固定了使用硅溶胶-凝胶基质的石墨烯-葡萄糖氧化酶。进行了伏安测量以定量评估将石墨烯片作为电极掺杂剂的适用性，并将其性能与单壁碳纳米管（SWCNT）进行了比较。生物燃料电池的阴极以类似的方法构建，只是共同固定了胆红素氧化酶。最后，构建了两个无膜酶促生物燃料电池，一个使用石墨烯片，另一个使用 SWCNT，并对它们的性能进行了比较。比较后，基于石墨烯的生物燃料电池表现出约 24.3+/-4 微瓦的最大功率密度（N=3），几乎是 SWCNT 生物燃料电池的两倍，并且石墨烯生物燃料电池的性能持续了 7 天。
