Alata Horticultural Research Institute, Mersin, Erdemli 33740, Turkey.
Bioresour Technol. 2010 May;101(9):3164-9. doi: 10.1016/j.biortech.2009.12.011. Epub 2010 Jan 6.
Pleurotus ostreatus and Pleurotus sajor-caju were studied for their ability to produce laccase and carboxymethylcellulase (CMCase) enzymes on different agricultural wastes under solid state fermentation. The spawns of P. ostreatus and P. sajor-caju were inoculated on different agricultural wastes including viticulture wastes, wheat straw, paddy straw, sesame straw, sawdust as well as the mixtures of these wastes with wheat bran. The carbon and nitrogen contents of substrates containing bran were the highest. The laccase activities of P. ostreatus and P. sajor-caju reached the highest values on the day 10 of mycelial growth. This enzyme activity was higher on the substrates containing bran which had high nitrogen and low C/N ratio than the other tested substrates with no bran. The CMCase activities of P. sajor-caju and P. ostreatus had two peaks on the 5th day of mycelial growth and after first flash. P. ostreatus and P. sajor-caju grown on substrates containing wheat bran had higher biological efficiencies and total yields as well as higher CMCase and laccase activities.
糙皮侧耳菌和美味侧耳菌被研究其在固态发酵条件下利用不同农业废弃物产漆酶和羧甲基纤维素酶(CMCase)的能力。糙皮侧耳菌和美味侧耳菌的孢子接种在不同的农业废弃物上,包括葡萄栽培废弃物、麦秸、稻秸、芝麻秸、木屑以及这些废弃物与麦麸的混合物。含有麸皮的基质的碳和氮含量最高。糙皮侧耳菌和美味侧耳菌的漆酶活性在菌丝生长第 10 天达到最高值。与其他不含麸皮的测试基质相比,含有高氮和低 C/N 比的麸皮基质上的酶活性更高。美味侧耳菌和糙皮侧耳菌的 CMCase 活性在菌丝生长第 5 天和第一次闪光后有两个峰值。在含有麦麸的基质上生长的糙皮侧耳菌和美味侧耳菌具有更高的生物效率和总产量,以及更高的 CMCase 和漆酶活性。