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本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
O2 and reactive oxygen species detoxification complex, composed of O2-responsive NADH:rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum.丙酮丁醇梭菌中由氧气响应型NADH：红素氧还蛋白氧化还原酶-黄素蛋白A2-脱硫铁氧化还原蛋白操纵子酶、rubperoxin和红素氧还蛋白组成的氧气和活性氧解毒复合物。
Appl Environ Microbiol. 2009 Feb;75(4):1021-9. doi: 10.1128/AEM.01425-08. Epub 2009 Jan 5.
3
Pathway for H2O2 and O2 detoxification in Clostridium acetobutylicum.丙酮丁醇梭菌中H2O2和O2解毒途径。
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4
An O2-inducible rubrerythrin-like protein, rubperoxin, is functional as a H2O2 reductase in an obligatory anaerobe Clostridium acetobutylicum.一种氧气诱导型类红素蛋白——rubperoxin，在专性厌氧菌丙酮丁醇梭菌中作为过氧化氢还原酶发挥作用。
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5
RIKEN structural genomics beamlines at the SPring-8; high throughput protein crystallography with automated beamline operation.日本理化学研究所（RIKEN）在SPring-8的结构基因组学光束线；通过自动光束线操作实现高通量蛋白质晶体学。
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6
Adaptive responses to oxygen stress in obligatory anaerobes Clostridium acetobutylicum and Clostridium aminovalericum.丙酮丁醇梭菌和氨基戊酸梭菌这两种专性厌氧菌对氧应激的适应性反应。
Appl Environ Microbiol. 2005 Dec;71(12):8442-50. doi: 10.1128/AEM.71.12.8442-8450.2005.
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Beamline Scheduling Software: administration software for automatic operation of the RIKEN structural genomics beamlines at SPring-8.光束线调度软件：用于日本理化学研究所（RIKEN）在SPring-8的结构基因组学光束线自动运行的管理软件。
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9
Identification of O2-induced peptides in an obligatory anaerobe, Clostridium acetobutylicum.在专性厌氧菌丙酮丁醇梭菌中鉴定氧气诱导的肽段。
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10
Purification and characterization of an H2O-forming NADH oxidase from Clostridium aminovalericum: existence of an oxygen-detoxifying enzyme in an obligate anaerobic bacteria.来自氨基戊酸梭菌的产H2O的NADH氧化酶的纯化与表征：专性厌氧菌中存在氧解毒酶
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丙酮丁醇梭菌中NADH：铁氧化还原蛋白氧化还原酶的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of NADH:rubredoxin oxidoreductase from Clostridium acetobutylicum.

作者信息

Nishikawa Koji, Shomura Yasuhito, Kawasaki Shinji, Niimura Youichi, Higuchi Yoshiki

机构信息

Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):23-5. doi: 10.1107/S1744309109047162. Epub 2009 Dec 25.

DOI:10.1107/S1744309109047162
PMID:20057062
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2805528/
Abstract

NADH

rubredoxin oxidoreductase (NROR), an O(2)-inducible protein, is a versatile electron donor for scavengers of O(2) and reactive oxygen species (ROS) in Clostridium acetobutylicum. Recombinant NROR was overexpressed in Escherichia coli and purified to homogeneity; it was subsequently crystallized using the sitting-drop vapour-diffusion method at 293 K. Preliminary crystallographic analysis revealed that the crystals belonged to space group P4(1)22 or P4(3)22, with unit-cell parameters a = b = 98.6, c = 88.3 A, and diffracted to 2.1 A resolution. Assuming that the crystals contained one molecule per asymmetric unit, the Matthews coefficient was calculated to be 2.7 A(3) Da(-1) and the solvent content to be 54.1%.

摘要

NADH

铁氧化还原蛋白氧化还原酶（NROR）是一种受O₂诱导的蛋白质，是丙酮丁醇梭菌中O₂和活性氧（ROS）清除剂的通用电子供体。重组NROR在大肠杆菌中过表达并纯化至同质；随后在293K下使用坐滴气相扩散法进行结晶。初步晶体学分析表明，晶体属于空间群P4(1)22或P4(3)22，晶胞参数a = b = 98.6，c = 88.3 Å，衍射分辨率为2.1 Å。假设每个不对称单元包含一个分子，计算出的马修斯系数为2.7 ų Da⁻¹，溶剂含量为54.1%。