来自大肠杆菌的ytfG基因产物的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli.
作者信息
Kim In-Kwon, Yim Hyung-Soon, Kim Dong-Won, Kim Young-Min, Lee Heung-Soo, Cha Sun-Shin, Kang Sa-Ouk
机构信息
Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):365-7. doi: 10.1107/S0907444903027781. Epub 2004 Jan 23.
The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 A data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 A. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.
具有NAD(P)H：醌氧化还原酶活性的大肠杆菌ytfG基因产物，于296 K通过悬滴气相扩散法结晶。利用韩国浦项光源的同步辐射收集了一套1.78 Å的数据集。该晶体属于原始三角晶系，晶胞参数a = b = 81.7，c = 76.8 Å。堆积密度分析表明，不对称单元可能包含一个单体，溶剂含量为48.8%。
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