Graduate School of Science & Technology, Kumamoto University, Kumamoto 860-8555, Japan.
Water Sci Technol. 2010;61(1):119-26. doi: 10.2166/wst.2010.796.
Anaerobic ammonium oxidation (anammox) is a novel nitrogen pathway catalyzed by anammox bacteria which are obligate anaerobic chemoautotrophs. In this study, enrichment culture of marine anammox bacteria (MAAOB) from the samples related to seawater was conducted. Simultaneous removal of ammonium and nitrite was confirmed in continuous culture inoculated with sediment of a sea-based waste disposal site within 50 days. However, no simultaneous nitrogen removal was observed in cultures inoculated with seawater-acclimated denitrifying sludge or with muddy sediment of tideland even during 200 days. Nitrogen removal rate of 0.13 kg/m(3)/day was achieved at nitrogen loading rate of 0.16 kg/m(3)/day after 320th days in the culture inoculated with the sediment of waste disposal site. The nitrogen removal ratio between ammonium nitrogen and nitrite nitrogen was 1:1.07. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that an abundance of the bacteria close to MAAOB and coexistence of ammonium oxidizing bacteria and denitrifying bacteria in the culture.
厌氧氨氧化（anammox）是一种新型的氮代谢途径，由厌氧氨氧化菌（anammox bacteria）催化，这些细菌是专性厌氧的化能自养菌。本研究从与海水相关的样品中进行了海洋厌氧氨氧化菌（MAAOB）的富集培养。在接种了来自海上废物处理场的沉积物的连续培养中，在 50 天内确认了同时去除铵和亚硝酸盐。然而，在接种了海水驯化反硝化污泥或滩涂淤泥的培养物中，即使在 200 天内，也没有观察到同时的氮去除。在接种废物处理场沉积物的培养物中，在第 320 天时，在氮负荷为 0.16 kg/m³/天时，实现了 0.13 kg/m³/天的氮去除率。铵氮和亚硝酸盐氮的氮去除比为 1:1.07。变性梯度凝胶电泳（DGGE）分析表明，培养物中存在大量接近 MAAOB 的细菌，以及氨氧化菌和反硝化菌的共存。
