3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Ave Park, 4806-909 Taipas, Guimarães, Portugal.
J Tissue Eng Regen Med. 2010 Jul;4(5):349-55. doi: 10.1002/term.245.
Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.
骨形态发生蛋白（BMPs）是具有强烈促进新骨形成能力的细胞因子。在此，我们报告了使用丝素蛋白微球作为载体来递送 BMP-2、BMP-9 或 BMP-14。通过使用乙醇逐滴添加的温和方法制备了含 BMP 的丝素蛋白微球，其平均直径为 2.7 +/- 0.3 微米。根据负载的 BMP 的类型和数量，包封效率在 67.9 +/- 6.1%至 97.7 +/- 2.0%之间变化。释放动力学表明，BMP-2、BMP-9 和 BMP-14 以两相释放曲线释放，在最初两天有一个突释，然后是一个较慢的释放，持续 14 天。释放数据最好通过 Korsmeyer 模型和药物扩散的 Fickian 模型来解释。丝素蛋白微球可为组织工程应用中不同 BMP 的持续递送提供一种有前途的方法。
