Malkevich Nina, McCarron Richard M, Mahon Richard T
Trauma & Resuscitative Medicine Department, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA.
Aviat Space Environ Med. 2010 Jan;81(1):15-21. doi: 10.3357/asem.2556.2010.
The use of hyperbaric oxygen (HBO) to expedite decompression from saturation has not been proven and may increase risk of toxicity to the pulmonary system. To evaluate any benefit of HBO during decompression, we used a 70-kg swine model of saturation and examined lung tissue by microarray analysis for evidence of RNA regulation.
Unrestrained, non-sedated swine were compressed to 132 fsw (5 ATA) for 22 h to achieve saturation. Animals then underwent decompression on air (AirD) or HBO (HBOD) starting at 45 fsw (2.36 ATA). Animals were evaluated for Type I and Type II decompression sickness (DCS) for 24 h. Control (SHAM) animals were placed in the chamber for the same duration, but were not compressed. Animals were sacrificed 24 h after exposure and total RNA was isolated from lung samples for microarray hybridizations on the Affymetrix platform.
There was no evidence of Type I DCS or severe cardiopulmonary DCS in any of the animals; abnormal gaits were noted only in the HBOD group (4/9).Three genes (nidogen 2, calcitonin-like receptor, and pentaxin-related gene) were significantly up-regulated in both the AirD and HBOD groups compared to controls. Three other genes (TN3, platelet basic protein, and cytochrome P450) were significantly down-regulated in both groups.
HBO during decompression from saturation did not reduce the incidence of DCS. Gene regulation was apparent and similar in both the AirD and HBOD groups, particularly in genes related to immune function and cell signaling.
使用高压氧（HBO）来加速从饱和状态减压尚未得到证实，且可能增加对肺部系统的毒性风险。为了评估减压过程中HBO的任何益处，我们使用了70千克的饱和猪模型，并通过微阵列分析检查肺组织以寻找RNA调节的证据。
将未受约束、未镇静的猪压缩至132英尺海水深度（5个绝对大气压）并保持22小时以达到饱和状态。然后，动物从45英尺海水深度（2.36个绝对大气压）开始在空气环境下减压（空气减压组，AirD）或在HBO环境下减压（HBO减压组，HBOD）。对动物进行24小时的I型和II型减压病（DCS）评估。对照（假手术，SHAM）组动物在舱内放置相同时间，但未进行压缩。暴露24小时后处死动物，从肺样本中分离总RNA，用于在Affymetrix平台上进行微阵列杂交。
在任何动物中均未发现I型DCS或严重心肺DCS的证据；仅在HBOD组（4/9）观察到异常步态。与对照组相比，AirD组和HBOD组中三个基因（巢蛋白2、降钙素样受体和五聚素相关基因）均显著上调。另外三个基因（TN3、血小板碱性蛋白和细胞色素P450）在两组中均显著下调。
从饱和状态减压过程中使用HBO并未降低DCS的发生率。基因调节在AirD组和HBOD组中均很明显且相似，特别是在与免疫功能和细胞信号传导相关的基因中。
