Research Institute for the Biology of Farm Animals, RU Nutritional Physiology 'Oskar Kellner', Dummerstorf, Germany.
FEMS Microbiol Ecol. 2010 Apr;72(1):132-42. doi: 10.1111/j.1574-6941.2009.00826.x. Epub 2009 Dec 7.
The effects of inulin and alginate on intestinal microbial ecophysiology were investigated in piglets fed a diet (C) with 0.1% alginate (C+A) or 1.5% inulin (C+I) from weaning at day 28. The experiment was performed at an experimental farm (EF) and a commercial farm (CF). Digesta was collected from the ileum, caecum and colon of four piglets from each group on days 29, 30, 33 and 39. The metabolite concentrations changed with age. Colonic and caecal metabolites were affected by prebiotic treatment. Changes in microbiota composition were assessed by cultivation and denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Enterococci increased in C+A at EF and decreased in C+I at both farms. Lactobacilli decreased in all segments in the experimental groups on days 30 and 33. Yeasts in C+I were five times lower at CF than at EF on day 39. The richness and diversity of DGGE profiles increased in the experimental groups. The evenness of colon digesta-derived DGGE profiles was higher in the experimental groups than in C and this situation was reversed in the distal small intestine. Multivariate redundancy analysis confirmed the recorded effects. In summary, both prebiotics affected the intestinal microbiota, and the changes were more pronounced at the CF.
本研究旨在探讨饲粮中添加 0.1%海藻酸钠（C+A）或 1.5%菊糖（C+I）对 28 日龄断奶仔猪肠道微生物生态生理学的影响。试验分别在实验农场（EF）和商业农场（CF）进行。于第 29、30、33 和 39 天，从每组 4 头仔猪的回肠、盲肠和结肠中采集食糜。代谢物浓度随日龄变化而变化。前处理可改变结肠和盲肠代谢物。采用培养和变性梯度凝胶电泳（DGGE）技术对 16S rRNA 基因片段进行分析，评估微生物群落组成的变化。在 EF，C+A 组中肠球菌增加，而在 CF，C+I 组中肠球菌减少。在试验组中，所有段的乳酸杆菌在第 30 和 33 天减少。在 CF，C+I 组的酵母在第 39 天比 EF 低 5 倍。DGGE 图谱的丰富度和多样性在试验组中增加。与 C 组相比，试验组结肠食糜 DGGE 图谱的均匀度更高，而在远端小肠中则相反。冗余分析证实了所记录的影响。总之，两种益生元都影响了肠道微生物群，而且在 CF 中的影响更为明显。
