决明子多糖对仔猪肠道微生物区系的影响。
Effect of polysaccharides of cassiae seeds on the intestinal microflora of piglets.
作者信息
Deng Ze-yuan, Zhang Jin-wu, Li Jing, Fan Ya-wei, Cao Shu-wen, Huang Rui-lin, Yin Yu-long, Zhong Hua-yi, Li Tie-jun
机构信息
Key Laboratory of Food Science, Department of Food Science and Engineering, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, PR China.
出版信息
Asia Pac J Clin Nutr. 2007;16 Suppl 1:143-7.
The objective of this study was to examine if polysaccharides from Cassiae Seeds (PCS) can be used as prebiotics to improve the intestinal microflora of piglets with an in vitro and an in vivo trial. The in vitro trial was conducted to study the dose-response effect of PCS on the growth of E. coli 09 and Lactobacillus with traditional plate count method. The gradient culture mediums, containing 3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, 0.025 and 0% PCS, were inoculated with E. coli09, Lactobacillus and cecum content, respectively. PCS had no influence on the growth of E. coli09 from rejuvenation fluid, but inhibited the growth of E. coli09 from cecum content when the concentration of PCS was higher than 0.1%. Lactobacillus counts were significantly increased with 0.1% PCS or higher (p< 0.05); and the largest increase was found with 0.8% PCS. With the inoculum of cecum content in the medium, Lactobacillus counts increased when the concentration of PCS was 0.4% and 0.8%, whilst E. coli 09 counts decreased. The in vivo trial was carried out to investigate the effect of PCS on the growth of E. coli 09 and Lactobacillus in piglets. Thirty six barrows (average initial BW = 6.5 kg) were randomly divided into 3 groups with 6 each, fed diets supplemented without or with 0.4% or 0.8% PCS. After 14 days, 3 piglets were slaughtered from each group; digesta samples were collected from the ileum, cecum and colon for detection of E.coli 09 and Lactobacillus with plate count method. Samples of the tissue and content of the cecum were taken for detection of caecal microflora profiles with Denaturing Gradient Gel Electrophoresis (DGGE) technique. The dietary inclusion of PCS increased Lactobacillus counts, but reduced E. coli 09 counts in digesta of ileum, cecum and colon of piglets. The dietary inclusion of 0.8% PCS significantly increased the number of electrophoresis brands of caecal bacterial microflora in mucosa and content of the cecum (p< 0.05). These results confirmed the dynamic change in the intestinal microflora profile with the dietary inclusion of PCS in piglets. Thus, PCS can be used as prebiotics to improve the intestinal microflora.
本研究的目的是通过体外和体内试验，检验决明子多糖（PCS）是否可用作益生元来改善仔猪的肠道微生物群。体外试验采用传统平板计数法，研究PCS对大肠杆菌09和乳酸杆菌生长的剂量反应效应。分别用含有3.2%、1.6%、0.8%、0.4%、0.2%、0.1%、0.05%、0.025%和0% PCS的梯度培养基接种大肠杆菌09、乳酸杆菌和盲肠内容物。PCS对复苏液中大肠杆菌09的生长没有影响，但当PCS浓度高于0.1%时，可抑制盲肠内容物中大肠杆菌09的生长。当PCS浓度为0.1%及以上时，乳酸杆菌数量显著增加（p<0.05）；其中，0.8% PCS组的增加幅度最大。在培养基中接种盲肠内容物后，当PCS浓度为0.4%和0.8%时，乳酸杆菌数量增加，而大肠杆菌09数量减少。体内试验旨在研究PCS对仔猪体内大肠杆菌09和乳酸杆菌生长的影响。将36头公猪（平均初始体重=6.5千克）随机分为3组，每组6头，分别饲喂不添加PCS、添加0.4% PCS或0.8% PCS的日粮。14天后，每组宰杀3头仔猪；采集回肠、盲肠和结肠的食糜样本，用平板计数法检测大肠杆菌09和乳酸杆菌。采集盲肠组织和内容物样本，采用变性梯度凝胶电泳（DGGE）技术检测盲肠微生物群谱。日粮中添加PCS可增加仔猪回肠、盲肠和结肠食糜中乳酸杆菌数量，但减少大肠杆菌09数量。日粮中添加0.8% PCS可显著增加盲肠黏膜和内容物中盲肠细菌微生物群的电泳条带数量（p<0.05）。这些结果证实了日粮中添加PCS后仔猪肠道微生物群谱的动态变化。因此，PCS可用作益生元来改善肠道微生物群。
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