从刺葡萄中分离得到的花色苷成分分析及其对 HT-29 人结肠癌细胞侵袭的抑制活性。
Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their anti-invasive activity on HT-29 human colon cancer cells.
机构信息
Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, South Korea.
出版信息
Food Chem Toxicol. 2010 Mar;48(3):903-9. doi: 10.1016/j.fct.2009.12.031. Epub 2010 Jan 6.
We isolated anthocyanins from fruits of Vitis coignetiae Pulliat, characterized the anthocyanin profile, and investigated the anti-invasive effects of the anthocyanins on human colon cancer cells. The anthocyanins inhibited cell invasion in a dose-dependent manner, as measured by Matrigel invasion assays, by suppression of matrix metalloproteinase (MMP)-2 and MMP-9 expression. The anti-invasive activity of the anthocyanins was associated with modulation of constitutive nuclear factor kappaB (NF-kappaB) activation. The activation of NF-kappaB triggered by tumor necrosis factor-alpha was also inhibited by the anthocyanins through suppression IkappaBalpha phosphorylation. AIMs inhibited the expression of NF-kappaB-regulated proteins. In conclusion, this study suggested that the anthocyanins isolated from fruits of V. coignetiae Pulliat should have anti-invasive activities on human colon cancer cells and the activities should be related to the inhibition of NF-kappaB-regulated proteins such as MMP-2 and MMP-9 expression through the inhibition of NF-kappaB activation.
我们从刺葡萄果实中分离出花色苷，对花色苷成分进行了鉴定，并研究了花色苷对人结肠癌细胞侵袭的抑制作用。通过基质胶侵袭实验，花色苷呈剂量依赖性抑制基质金属蛋白酶（MMP）-2 和 MMP-9 的表达，从而抑制细胞侵袭。花色苷的抗侵袭活性与调节固有核因子κB（NF-κB）激活有关。花色苷还通过抑制 IkappaBalpha 磷酸化来抑制肿瘤坏死因子-α诱导的 NF-κB 激活。AIM 抑制 NF-κB 调节蛋白的表达。总之，本研究表明，从刺葡萄果实中分离出的花色苷对人结肠癌细胞具有抗侵袭活性，其活性可能与通过抑制 NF-κB 激活抑制 NF-κB 调节蛋白（如 MMP-2 和 MMP-9）的表达有关。
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