Behrendt Inken, Röder Isabella, Will Frank, Mostafa Hamza, Gonzalez-Dominguez Raúl, Meroño Tomás, Andres-Lacueva Cristina, Fasshauer Mathias, Rudloff Silvia, Kuntz Sabine
Department of Nutritional Science, Human Nutrition, Justus-Liebig-University, 35390 Giessen, Germany.
Department of Beverage Research, Hochschule Geisenheim University, 65366 Geisenheim, Germany.
Antioxidants (Basel). 2022 Jul 8;11(7):1341. doi: 10.3390/antiox11071341.
Cancer mortality is mainly due to metastasis. Therefore, searching for new therapeutic agents suppressing cancer cell migration is crucial. Data from human studies regarding effects of anthocyanins on cancer progression, however, are scarce and it is unclear whether physiological concentrations of anthocyanins and their metabolites reduce cancer cell migration in vivo. In addition, interactions with chemotherapeutics like 5-fluorouracil (5-FU) are largely unknown. Thus, we combined a placebo-controlled, double-blinded, cross-over study with in vitro migration studies of colon cancer cell lines to examine the anti-migratory effects of plasma-isolated anthocyanins and their metabolites (PAM). Healthy volunteers ( = 35) daily consumed 0.33 L of an anthocyanin-rich grape/bilberry juice and an anthocyanin-depleted placebo juice for 28 days. PAM were isolated before and after intervention by solid-phase extraction. HT-29 and Caco-2 cells were incubated with PAM in a Boyden chamber. Migration of HT-29 cells was significantly inhibited by PAM from juice but not from placebo. In contrast, Caco-2 migration was not affected. Co-incubation with 5-FU and pooled PAM from volunteers ( = 10), which most effectively inhibited HT-29 migration, further reduced HT-29 migration in comparison to 5-FU alone. Therefore, PAM at physiological concentrations impairs colon cancer cell migration and may support the effectiveness of chemotherapeutics.
癌症死亡率主要归因于转移。因此，寻找抑制癌细胞迁移的新治疗药物至关重要。然而，关于花青素对癌症进展影响的人体研究数据稀缺，尚不清楚生理浓度的花青素及其代谢产物是否能在体内减少癌细胞迁移。此外，花青素与5-氟尿嘧啶（5-FU）等化疗药物的相互作用在很大程度上尚不明确。因此，我们将一项安慰剂对照、双盲、交叉研究与结肠癌细胞系的体外迁移研究相结合，以检验血浆分离的花青素及其代谢产物（PAM）的抗迁移作用。35名健康志愿者每天饮用0.33升富含花青素的葡萄/越橘汁和不含花青素的安慰剂果汁，持续28天。干预前后通过固相萃取分离PAM。HT-29和Caco-2细胞在博伊登小室中与PAM共同孵育。来自果汁而非安慰剂的PAM显著抑制了HT-29细胞的迁移。相比之下，Caco-2细胞的迁移未受影响。将5-FU与来自10名志愿者的合并PAM（其最有效地抑制了HT-29细胞迁移）共同孵育，与单独使用5-FU相比，进一步降低了HT-29细胞的迁移。因此，生理浓度的PAM会损害结肠癌细胞迁移，并可能增强化疗药物的疗效。
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