Department of Psychology, University of South Florida, 4202 E. Fowler Ave. PCD4118G, Tampa, FL 33620, USA.
Hear Res. 2010 Mar;261(1-2):36-41. doi: 10.1016/j.heares.2009.12.029. Epub 2010 Jan 7.
Neurons in the anteroventral cochlear nucleus (AVCN) of DBA/2J (D2) and C57BL/6J (B6) mice were immunohistochemically labeled for the calcium binding protein parvalbumin (PV). Prior to this, mice were treated for 12h nightly with a "high-frequency" augmented acoustic environment (HAAE: repetitive bursts of a 70 dB sound pressure level, half-octave noise band centered at 20 kHz). This was done during the period that hearing loss occurs: pre-weaning to 55 days in D2 mice and weaning to 9 months in B6 mice. After HAAE treatment in D2 mice, high-frequency hearing loss was ameliorated and fewer PV-labeled neurons were found in the AVCN compared to untreated controls. HAAE treatment in B6 mice exacerbated high-frequency hearing loss, yet the number of PV-labeled AVCN neurons in treated mice did not differ significantly from that of control mice. The findings suggest that HAAE treatment provides relief from physiological stress caused by deprivation of auditory input from the impaired cochlea.
DBA/2J（D2）和 C57BL/6J（B6）小鼠的前腹侧耳蜗核（AVCN）神经元通过钙结合蛋白 parvalbumin（PV）的免疫组织化学标记进行标记。在此之前，每晚用“高频”增强的声环境（HAAE：70 分贝声压水平的重复爆发，以 20 kHz 为中心的半倍频带噪声）对小鼠进行处理。这是在听力损失发生期间进行的：在 D2 小鼠中从断奶前到 55 天，在 B6 小鼠中从断奶到 9 个月。在 D2 小鼠中进行 HAAE 治疗后，高频听力损失得到改善，与未经处理的对照组相比，AVCN 中发现的 PV 标记神经元较少。在 B6 小鼠中进行 HAAE 治疗会加剧高频听力损失，但处理组小鼠的 AVCN 中 PV 标记神经元的数量与对照组小鼠没有显著差异。这些发现表明，HAAE 治疗缓解了因受损耳蜗听觉输入剥夺而引起的生理应激。
