纤溶酶原激活物抑制剂-1晶体的初步X射线分析。

Preliminary X-ray analysis of crystals of plasminogen activator inhibitor-1.

作者信息

Goldsmith E J, Sheng-Cheng C, Danley D E, Gerard R D, Geoghegan K F, Mottonen J, Strand A

机构信息

Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050.

出版信息

Proteins. 1991;9(3):225-7. doi: 10.1002/prot.340090308.

DOI:10.1002/prot.340090308

PMID:2006140

Abstract

Crystals of bacterially expressed plasminogen activator inhibitor (PAI-1) suitable for X-ray diffraction analysis have been obtained from 8% (w/v) PEG 1500, pH 8.25. The space group is P1, and the lattice constants are a = 82.17 A, b = 47.82 A, c = 62.89 A, alpha = 90.00 degrees, beta = 106.90 degrees, gamma = 106.84 degrees. The diffraction limit is 2.3 A, and the unit cell contains two molecules of PAI-1. The crystals contain latent PAI-1 which can be partly reactivated by exposure to denaturants.

摘要

已从8%（w/v）聚乙二醇1500、pH 8.25中获得适合X射线衍射分析的细菌表达纤溶酶原激活物抑制剂（PAI-1）晶体。空间群为P1，晶格常数为a = 82.17 Å，b = 47.82 Å，c = 62.89 Å，α = 90.00°，β = 106.90°，γ = 106.84°。衍射极限为2.3 Å，晶胞包含两个PAI-1分子。这些晶体含有潜在的PAI-1，通过暴露于变性剂可部分重新激活。