嗜热栖热放线菌木聚糖酶Z催化结构域的结晶及初步衍射分析

Crystallization and preliminary diffraction analysis of the catalytic domain of xylanase Z from Clostridium thermocellum.

作者信息

Souchon H, Spinelli S, Béguin P, Alzari P M

机构信息

Unité d'Immunologie Structurale, URA 359 CNRS, Institut Pasteur, Paris, France.

出版信息

J Mol Biol. 1994 Jan 28;235(4):1348-50. doi: 10.1006/jmbi.1994.1089.

DOI:10.1006/jmbi.1994.1089

PMID:8308898

Abstract

The catalytic domain of a thermostable xylanase from Clostridium thermocellum has been expressed in Escherichia coli and crystallized from a polyethylene glycol 2000 solution by the hanging drop method. Crystals belong to the triclinic space group P1 with cell dimensions a = 46.8 A, b = 50.8 A, c = 70.3 A, alpha = 100.7 degrees, beta = 83.8 degrees, gamma = 101.6 degrees, and two molecules in the unit cell. These crystals diffract X-rays to at least 1.8 A resolution and are suitable for high-resolution X-ray analysis.

摘要

来自热纤梭菌的一种耐热木聚糖酶的催化结构域已在大肠杆菌中表达，并通过悬滴法从聚乙二醇2000溶液中结晶。晶体属于三斜晶系空间群P1，晶胞参数为a = 46.8 Å，b = 50.8 Å，c = 70.3 Å，α = 100.7°，β = 83.8°，γ = 101.6°，晶胞中有两个分子。这些晶体的X射线衍射分辨率至少为1.8 Å，适合进行高分辨率X射线分析。