伊朗阿瓦士献血者中丙型肝炎病毒基因型的测定

Determination of hepatitis C virus genotypes among blood donors in Ahvaz, Iran.

作者信息

Farshadpour F, Makvandi M, Samarbafzadeh A R, Jalalifar M A

机构信息

Department of Virology, Faculty of Medicine and Tropical and Infectious Disease Research Centre, Ahvaz Jondishapour University of Medical Sciences, Golestan Blved, Ahvaz, Iran.

出版信息

Indian J Med Microbiol. 2010 Jan-Mar;28(1):54-6. doi: 10.4103/0255-0857.58731.

DOI:10.4103/0255-0857.58731

PMID:20061766

Abstract

This study aims to determine the genotypes of hepatitis C virus (HCV) among blood donors at Ahvaz Blood Transfusion Centre. Blood samples were taken from 2376 blood donors - 1795 (75.54%) male and 581(24.45%) female - who referred to Ahvaz Blood Transfusion Centre during 2007-2008. Detection of anti-HCV antibody for all the donors was carried out by ELISA and the confirmatory RIBA tests. HCV RT-PCR followed by RFLP test was carried out for anti-HCV positive samples. Out of 2376 blood donors, only 55 (2.3%) male donors showed to be positive for HCV antibody by ELISA and RIBA tests out of which 45(1.8%) donors were positive for RT-PCR test. Female donors were negative for HCV antibody. The result of HCV genotyping by RFLP test showed 24 (53.3%) for 1a, 17 (37.7%) for 3a (a) and 4 (8.8%) for 3a (b) genotypes respectively. In conclusion, high prevalence of 53.3% HCV 1a genotype was observed among blood donors in Ahvaz city.

摘要

本研究旨在确定阿瓦士输血中心献血者中丙型肝炎病毒（HCV）的基因型。采集了2007年至2008年期间前往阿瓦士输血中心的2376名献血者的血样，其中男性1795名（75.54%），女性581名（24.45%）。通过酶联免疫吸附测定（ELISA）和确证免疫印迹分析（RIBA）检测所有献血者的抗HCV抗体。对抗HCV阳性样本进行HCV逆转录聚合酶链反应（RT-PCR），随后进行限制性片段长度多态性（RFLP）检测。在2376名献血者中，ELISA和RIBA检测显示只有55名（2.3%）男性献血者HCV抗体呈阳性，其中45名（1.8%）献血者RT-PCR检测呈阳性。女性献血者HCV抗体呈阴性。RFLP检测的HCV基因分型结果显示，1a型为24例（53.3%），3a（a）型为17例（37.7%），3a（b）型为4例（8.8%）。总之，在阿瓦士市献血者中观察到HCV 1a基因型的高流行率为53.3%。