Serebrennikova Yulia M, Patel Janus, Garcia-Rubio Luis H
College of Public Health, University of South Florida, 13201 Bruce B. Downs Boulevard, Tampa, Florida 33830, USA.
Appl Opt. 2010 Jan 10;49(2):180-8. doi: 10.1364/AO.49.000180.
The absorption and scattering properties of three developmental stages of protozoan parasite Plasmodium falciparum were studied both experimentally and theoretically. Experimentally, the light attenuation and forward scattering from parasites extracted from host erythrocyte cultures were measured with UV-visible spectroscopy. The measured spectra were interpreted theoretically with a model based on the core-shell Mie theory in terms of the structural and compositional characteristics of the protozoa. The model accurately reproduced the features of the measured spectra of all developmental stages. The results show that realistic quantitative estimates of the parasite size, nucleotide, and hemozoin contents can be derived from the UV-visible spectroscopy measurements.
对疟原虫恶性疟原虫三个发育阶段的吸收和散射特性进行了实验和理论研究。在实验中，使用紫外可见光谱法测量了从宿主红细胞培养物中提取的寄生虫的光衰减和前向散射。根据基于核壳米氏理论的模型，从原生动物的结构和组成特征方面对测量光谱进行了理论解释。该模型准确地再现了所有发育阶段测量光谱的特征。结果表明，可以从紫外可见光谱测量中得出对寄生虫大小、核苷酸和疟原虫色素含量的实际定量估计。
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