Masilamani Vadivel, Devanesan Sandhanasamy, Ravikumar Mani, Perinbam Kantharaj, AlSalhi Mohamad Saleh, Prasad Saradh, Palled Siddanna, Ganesh Kadirampatti Mani, Alsaeed Abbas Habeeb
Diagn Pathol. 2014 Oct 17;9:182. doi: 10.1186/s13000-014-0182-z.
Malaria is the most common disease transmitted by the bite by an infected female anopheles mosquito and caused by the plasmodium parasite. It is mostly prevalent in subtropical regions receiving abundant rain and supporting copious mosquito breeding. This disease is generally detected by the microscopic examination of blood films or antigen based rapid diagnostic test. Only occasionally the parasite DNA is detected using polymerase chain reaction in certain advanced, expensive laboratories.
An innovative spectral detection method based on the fluorescence spectra of a set of blood plasma biomolecules [tyrosine, tryptophan, nicotinamide adenine dinucleotide (NAD), and flavin adenine dinucleotide (FAD)] and red blood cell (RBC)-associated porphyrin is being evolved by our group.
The research so far has exhibited sensitivity and specificity values exceeding 90% based on the spectral features of blood components of 14 malaria patients and 20 numbers of age adjusted normal controls. The fluorescent biomolecules go out of proportion when the malarial parasite breaks down the hemoglobin of blood.
This technique has the potential to be used as an alternative diagnostic procedure for malaria since the instrumentation involved is portable and inexpensive.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_182.
疟疾是由受感染的雌性按蚊叮咬传播、由疟原虫寄生虫引起的最常见疾病。它主要流行于降雨丰富、利于大量蚊子繁殖的亚热带地区。这种疾病通常通过血液涂片的显微镜检查或基于抗原的快速诊断测试来检测。只有在某些先进、昂贵的实验室中，偶尔才会使用聚合酶链反应检测寄生虫DNA。
我们团队正在研发一种基于一组血浆生物分子[酪氨酸、色氨酸、烟酰胺腺嘌呤二核苷酸（NAD）和黄素腺嘌呤二核苷酸（FAD）]以及红细胞（RBC）相关卟啉荧光光谱的创新光谱检测方法。
基于14名疟疾患者和20名年龄匹配的正常对照者血液成分的光谱特征，迄今为止的研究显示敏感性和特异性值超过90%。当疟原虫分解血液中的血红蛋白时，荧光生物分子会失衡。
由于所涉及的仪器便携且价格低廉，这项技术有潜力用作疟疾的替代诊断程序。
本文的虚拟切片可在此处找到：http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_182 。
