Michalska-Szymaszek Małgorzata
Wojewódzka Stacja Sanitamrno-Epidemiologiczna w Rzeszowie, Oddział Laboratoryjny w Tarnobrzegu.
Rocz Panstw Zakl Hig. 2009;60(3):229-33.
E. coli O157 strains isolated from surface water and from a sick human were tested for their ability to survive in environment with pH < or = 7. Examinations were carried on TSB broth with pH range < or = 7 (modified with 1 M HCl) in temperatures 5 degrees C, 25 degrees C, 36 degrees C after 5 hours and 24 hours. It was shown that some of E. coli O157 strains have the survival ability in acid medium pH = 2 and 3 within 5 h in temperatures 5 degrees C, 25 degrees C, 36 degrees C. E. coli O157 strain, of human origin, grows at pH > 4 in temp. 25 degrees C and strain E. coli O157 from water at pH = 4 in this temperature. The acid tolerance of E. coli O157 suggested that the strain be came pathogenic for humans.
对从地表水和患病人类身上分离出的大肠杆菌O157菌株进行了测试，以检测它们在pH≤7的环境中的生存能力。在5℃、25℃、36℃下，于pH范围≤7（用1M盐酸调节）的胰酪大豆胨肉汤（TSB肉汤）中进行检测，分别在5小时和24小时后观察结果。结果表明，部分大肠杆菌O157菌株在5℃、25℃、36℃的温度下，于pH = 2和3的酸性培养基中5小时内具有生存能力。源自人类的大肠杆菌O157菌株在25℃下pH>4时生长，而来自水的大肠杆菌O157菌株在该温度下pH = 4时生长。大肠杆菌O157的耐酸性表明该菌株对人类具有致病性。
