Tsui Stephen K W, Fong Nga-Yin, Li Sai-Kam, Leung Ka-Kit, Chan Denise P C, Chan Paul K S, Wong Ka-Hing, Lee Shui-Shan
Centre for Microbial Genomics and Proteomics, Hong Kong SAR.
AIDS Res Hum Retroviruses. 2010 Jan;26(1):117-22. doi: 10.1089/aid.2009.0104.
With considerable capacity for genetic diversification, new HIV-1 genotypes have been reported over the years. Three HIV-1 isolates previously genotyped as B using gag and env sequences were completely sequenced and reanalyzed. Several amino acid mutations were found in vif, rev, and nef genes but not in gag or env sequences. These alterations have not previously been reported in Hong Kong. The investigation of phylogenetic relatedness revealed that a region of the vif of the studied Hong Kong isolates subtype B cluster contains several subtype D signature amino acid residues. Several unique mutations on vif in these three isolates were also identified.
由于具有相当大的基因多样化能力，多年来已报道了新的HIV-1基因型。对之前使用gag和env序列基因分型为B型的三株HIV-1分离株进行了全序列测定和重新分析。在vif、rev和nef基因中发现了几个氨基酸突变，但在gag或env序列中未发现。这些改变此前在香港尚未有报道。系统发育相关性研究表明，所研究的香港B亚型分离株群的vif区域包含几个D亚型特征性氨基酸残基。还鉴定出这三株分离株vif上的几个独特突变。
