Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, Kwandong University, College of Medicine, Seoul, Korea.
Am J Reprod Immunol. 2010 Feb;63(2):173-80. doi: 10.1111/j.1600-0897.2009.00777.x.
We aimed to investigate if peripheral blood natural killer (pNK) cell levels are correlated with decidual NK (dNK) cell levels, and if chemokine expression has any role in dNK cell regulation.
of study Decidual tissues of women having two or more miscarriages with normal karyotype were collected after miscarriage and an immuno-histochemisty study was made. pNK cells were evaluated using flow cytometric analysis.
The %CD3(-)/56(+) and %CD3(-)/56(+)/16(+) pNK cells showed a significant correlation with mean number of CD56(+) dNK cells. The number of decidual CD16(+) cells was significantly higher in women with elevated pNK (> or =15%) than that of normal pNK (<15%). The %CD3(-)/56(+) and %CD3(-)/56(+)/16(+) pNK cells showed an inverse correlation with duration of gestation. The CCL3(+) and CXCL12(+) cells were present in the decidua; however, staining intensity was not correlated with number of dNK cells.
The pNK cell levels reflect changes in dNK cell levels. This implicates that pNK cell level is a clinically useful marker to predict pregnancy outcome. Further study is needed to examine if elevated pNK cells enhance recruitment of dNK cells in the decidua.
我们旨在研究外周血自然杀伤 (pNK) 细胞水平是否与蜕膜自然杀伤 (dNK) 细胞水平相关，以及趋化因子表达是否在 dNK 细胞调节中起作用。
本研究收集了两名或多名有正常核型流产的女性的流产蜕膜组织，并进行了免疫组织化学研究。使用流式细胞术分析评估 pNK 细胞。
%CD3(-)/56(+) 和 %CD3(-)/56(+)/16(+) pNK 细胞与 CD56(+) dNK 细胞的平均数量呈显著相关。pNK（≥15%）升高的女性的蜕膜 CD16(+) 细胞数量明显高于 pNK 正常的女性（<15%）。%CD3(-)/56(+) 和 %CD3(-)/56(+)/16(+) pNK 细胞与妊娠时间呈负相关。CCL3(+) 和 CXCL12(+) 细胞存在于蜕膜中；然而，染色强度与 dNK 细胞数量无关。
pNK 细胞水平反映了 dNK 细胞水平的变化。这表明 pNK 细胞水平是预测妊娠结局的一种有用的临床标志物。需要进一步研究以检查升高的 pNK 细胞是否增强了 dNK 细胞在蜕膜中的募集。
