Institute of Zoology and Department of Life Science, National Taiwan University, Taipei, 10617, Taiwan, Republic of China.
Anim Genet. 2010 Aug;41(4):365-76. doi: 10.1111/j.1365-2052.2009.02014.x. Epub 2010 Jan 7.
The linkage maps of male and female tiger shrimp (P. monodon) were constructed based on 256 microsatellite and 85 amplified fragment length polymorphism (AFLP) markers. Microsatellite markers obtained from clone sequences of partial genomic libraries, tandem repeat sequences from databases and previous publications and fosmid end sequences were employed. Of 670 microsatellite and 158 AFLP markers tested for polymorphism, 341 (256 microsatellite and 85 AFLP markers) were used for genotyping with three F(1) mapping panels, each comprising two parents and more than 100 progeny. Chi-square goodness-of-fit test (chi(2)) revealed that only 19 microsatellite and 28 AFLP markers showed a highly significant segregation distortion (P < 0.005). Linkage analysis with a LOD score of 4.5 revealed 43 and 46 linkage groups in male and female linkage maps respectively. The male map consisted of 176 microsatellite and 49 AFLP markers spaced every approximately 11.2 cM, with an observed genome length of 2033.4 cM. The female map consisted of 171 microsatellite and 36 AFLP markers spaced every approximately 13.8 cM, with an observed genome length of 2182 cM. Both maps shared 136 microsatellite markers, and the alignment between them indicated 38 homologous pairs of linkage groups including the linkage group representing the sex chromosome. The karyotype of P. monodon is also presented. The tentative assignment of the 44 pairs of P. monodon haploid chromosomes showed the composition of forty metacentric, one submetacentric and three acrocentric chromosomes. Our maps provided a solid foundation for gene and QTL mapping in the tiger shrimp.
利用 256 个微卫星和 85 个扩增片段长度多态性（AFLP）标记，构建了雄性和雌性虎虾（P. monodon）的连锁图谱。微卫星标记来自部分基因组文库克隆序列、数据库串联重复序列和先前的出版物以及 fosmid 末端序列。在 670 个微卫星和 158 个 AFLP 标记中，有 341 个（256 个微卫星和 85 个 AFLP 标记）用于三个 F(1)作图群体的基因型分析，每个群体由两个亲本和 100 多个后代组成。卡方拟合优度检验（chi(2)）表明，只有 19 个微卫星和 28 个 AFLP 标记表现出高度显著的分离失真（P < 0.005）。LOD 得分 4.5 的连锁分析显示，雄性和雌性连锁图谱分别有 43 和 46 个连锁群。雄性图谱由 176 个微卫星和 49 个 AFLP 标记组成，间隔约 11.2 cM，观察到的基因组长度为 2033.4 cM。雌性图谱由 171 个微卫星和 36 个 AFLP 标记组成，间隔约 13.8 cM，观察到的基因组长度为 2182 cM。两个图谱共有 136 个微卫星标记，它们之间的比对表明有 38 对同源连锁群，包括代表性染色体的连锁群。还呈现了 P. monodon 的核型。44 对 P. monodon 单倍体染色体的暂定分配表明，由四十个中央着丝粒、一个亚中央着丝粒和三个近端着丝粒染色体组成。我们的图谱为虎虾中的基因和 QTL 作图提供了坚实的基础。
