Guo Liang, Xu Yu-Hui, Zhang Nan, Zhou Fa-Lin, Huang Jian-Hua, Liu Bao-Suo, Jiang Shi-Gui, Zhang Dian-Chang
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou, China.
Front Genet. 2019 Apr 9;10:326. doi: 10.3389/fgene.2019.00326. eCollection 2019.
The black tiger shrimp, , is important in both fishery and aquaculture and is the second-most widely cultured shrimp species in the world. However, the current strains cannot meet the market needs in various cultural environments, and the genome resources for are still lacking. Restriction-site associated DNA sequencing (RADseq) has been widely used in genetic linkage map construction and in quantitative trait loci (QTL) mapping. We constructed a high-density genetic linkage map with RADseq in a full-sib family. This map contained 6524 single nucleotide polymorphisms (SNPs) and 2208 unique loci. The total length was 3275.4 cM, and the genetic distance was estimated to be 1.1 Mb/cM. The sex trait is a dichotomous phenotype, and the same interval was detected as a QTL using QTL mapping and genome-wide association analysis. The most significant locus explained 77.4% of the phenotype variance. The sex locus was speculated to be the same in this species based on the sequence alignments in Mozambique, India, and Hawaii populations. The constructed genetic linkage map provided a valuable resource for QTL mapping, genome assembly, and genome comparison for shrimp. The demonstrated common sex locus is a step closer to locating the underlying gene.
斑节对虾在渔业和水产养殖中都很重要，是世界上第二大养殖对虾品种。然而，目前的品系无法满足不同养殖环境下的市场需求，并且斑节对虾的基因组资源仍然匮乏。限制性内切酶相关DNA测序（RADseq）已广泛应用于遗传连锁图谱构建和数量性状基因座（QTL）定位。我们利用RADseq在一个全同胞家系中构建了一张高密度遗传连锁图谱。该图谱包含6524个单核苷酸多态性（SNP）和2208个独特位点。图谱总长度为3275.4厘摩（cM），估计遗传距离为1.1兆碱基/厘摩（Mb/cM）。性别性状是一种二分表型，通过QTL定位和全基因组关联分析在同一区间检测到一个QTL。最显著的位点解释了77.4%的表型变异。根据莫桑比克、印度和夏威夷种群的序列比对推测该物种的性别位点相同。构建的遗传连锁图谱为对虾的QTL定位、基因组组装和基因组比较提供了宝贵资源。所证明的共同性别位点朝着定位潜在基因又迈进了一步。
