Rab35 介导吞噬作用过程中 Cdc42 和 Rac1 向质膜的运输。
Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis.
机构信息
Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul 120-749, South Korea.
出版信息
Mol Cell Biol. 2010 Mar;30(6):1421-33. doi: 10.1128/MCB.01463-09. Epub 2010 Jan 11.
Abstract
Phagocytosis of invading microbes requires dynamic rearrangement of the plasma membrane and its associated cytoskeletal actin network. The polarization of Cdc42 and Rac1 Rho GTPases to the site of plasma membrane protrusion is responsible for the remodeling of actin structures. However, the mechanism of Rho GTPase recruitment to these sites and the identities of accessory molecules involved in this process are not well understood. In this study, we uncovered several new components involved in innate immunity in Drosophila melanogaster. Our data demonstrate that Rab35 is a regulator of vesicle transport required specifically for phagocytosis. Moreover, recruitment of Cdc42 and Rac1 to the sites of filopodium and lamellipodium formation is Rab35 dependent and occurs by way of microtubule tracks. These results implicate Rab35 as the immune cell-specific regulator of vesicle transport within the actin-remodeling complex.
摘要
吞噬入侵微生物需要细胞质膜及其相关的肌动蛋白细胞骨架网络的动态重排。Cdc42 和 Rac1 Rho GTPases 向细胞质膜突出部位的极化负责肌动蛋白结构的重塑。然而，Rho GTPase 招募到这些部位的机制以及参与此过程的辅助分子的身份尚不清楚。在这项研究中，我们在黑腹果蝇中发现了几个参与先天免疫的新成分。我们的数据表明 Rab35 是囊泡运输的调节剂，对于吞噬作用是必需的。此外，Cdc42 和 Rac1 向丝状伪足和片状伪足形成部位的募集依赖于 Rab35，并且通过微管轨道发生。这些结果表明 Rab35 是肌动蛋白重塑复合物中囊泡运输的免疫细胞特异性调节剂。
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