Zdarova Karasova Jana, Novotny Ladislav, Antos Karel, Zivna Helena, Kuca Kamil
Department of Toxicology, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic.
Anal Sci. 2010 Jan;26(1):63-7. doi: 10.2116/analsci.26.63.
A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 +/- 1.71 microg/mL. The distribution of obidoxime was fast; the single maximum 23.62 +/- 3.563 microg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.
我们的研究提出了一种简单可靠的高效液相色谱法（HPLC），用于测定大鼠血浆中临床使用的乙酰胆碱酯酶（AChE）重活化剂（HI-6和双复磷）的水平。采用十八烷基硅胶固定相，以含5 mM辛烷磺酸钠和5 mM四甲基氯化铵（pH 2.3）的24%乙腈为流动相，通过HPLC进行分离。肌肉注射等摩尔剂量的两种肟（22.23 mg/kg）后，大鼠血浆中HI-6浓度在约20分钟时达到最大值，为15.26±1.71μg/mL。双复磷的分布很快；在约10分钟时记录到单一最大值23.62±3.563μg/mL。我们研究中提出的带紫外检测的HPLC是一种通用方法，可用于快速测定大鼠血浆中的双季铵AChE重活化剂。
