自闭症患者大脑中的 GABAAalpha4、alpha5、beta1 和 GABABR1 受体的 mRNA 和蛋白水平发生改变。
mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are altered in brains from subjects with autism.
机构信息
Department of Psychiatry, Division of Neuroscience Research, University of Minnesota School of Medicine, 420 Delaware St SE MMC 392, Minneapolis, MN 55455, USA.
出版信息
J Autism Dev Disord. 2010 Jun;40(6):743-50. doi: 10.1007/s10803-009-0924-z.
Abstract
We have shown altered expression of gamma-aminobutyric acid A (GABA(A)) and gamma-aminobutyric acid B (GABA(B)) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA(A) subunits previously associated with autism (GABRalpha4; GABRalpha5; GABRbeta1). Three GABA receptor subunits demonstrated mRNA and protein level concordance in superior frontal cortex (GABRalpha4, GABRalpha5, GABRbeta1) and one demonstrated concordance in cerebellum (GABBetaR1). These results provide further evidence of impairment of GABAergic signaling in autism.
摘要
我们已经在自闭症患者的大脑中发现了γ-氨基丁酸 A（GABA(A)）和γ-氨基丁酸 B（GABA(B)）受体表达的改变。在当前的研究中，我们试图通过 qRT-PCR 来验证我们的 GABA(B)R1 免疫印迹数据，并扩展我们之前的工作，以测量先前与自闭症相关的 3 种 GABA(A)亚基的 mRNA 和蛋白水平（GABRalpha4、GABRalpha5、GABRbeta1）。在前额皮质（GABRalpha4、GABRalpha5、GABRbeta1）中，3 种 GABA 受体亚基的 mRNA 和蛋白水平具有一致性，而在小脑（GABBetaR1）中则有 1 种具有一致性。这些结果为自闭症中 GABA 能信号传递受损提供了进一步的证据。
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