可视化 O-GlcNAc 水解酶的反应坐标。
Visualizing the reaction coordinate of an O-GlcNAc hydrolase.
机构信息
York Structural Biology laboratory, Department of Chemistry, The University of York, YO10 5YW, UK.
出版信息
J Am Chem Soc. 2010 Feb 17;132(6):1807-9. doi: 10.1021/ja9086769.
N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.
核细胞质蛋白丝氨酸和苏氨酸残基上的 N-乙酰氨基葡萄糖 β-O 连接（O-GlcNAc）与神经退行性变、细胞应激反应和转录调控有关。O-GlcNAcase（OGA）使用底物辅助催化机制催化 O-GlcNAc 的去除。在这里，我们使用化学方法定义反应坐标，并直接观察迈克尔加成复合物和恶唑啉中间体。
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