Suppr超能文献

丙型肝炎病毒 ARFP/Core+1/S 蛋白中固有无序的流行率。

Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein.

机构信息

Université de Strasbourg, CNRS FRE 3211, Ecole Supérieure de Biotechnologie de Strasbourg, Illkirch, France.

出版信息

FEBS J. 2010 Feb;277(3):774-89. doi: 10.1111/j.1742-4658.2009.07527.x. Epub 2010 Jan 7.

Abstract

The hepatitis C virus (HCV) Core+1/S polypeptide, also known as alternative reading frame protein (ARFP)/S, is an ARFP expressed from the Core coding region of the viral genome. Core+1/S is expressed as a result of internal initiation at AUG codons (85-87) located downstream of the polyprotein initiator codon, and corresponds to the C-terminal part of most ARFPs. Core+1/S is a highly basic polypeptide, and its function still remains unclear. In this work, untagged recombinant Core+1/S was expressed and purified from Escherichia coli in native conditions, and was shown to react with sera of HCV-positive patients. We subsequently undertook the biochemical and biophysical characterization of Core+1/S. The conformation and oligomeric state of Core+1/S were investigated using size exclusion chromatography, dynamic light scattering, fluorescence, CD, and NMR. Consistent with sequence-based disorder predictions, Core+1/S lacks significant secondary structure in vitro, which might be relevant for the recognition of diverse molecular partners and/or for the assembly of Core+1/S. This study is the first reported structural characterization of an HCV ARFP/Core+1 protein, and provides evidence that ARFP/Core+1/S is highly disordered under native conditions, with a tendency for self-association.

摘要

丙型肝炎病毒 (HCV) Core+1/S 多肽,也称为交替阅读框蛋白 (ARFP)/S,是一种从病毒基因组的 Core 编码区表达的 ARFP。Core+1/S 是由于位于多蛋白起始密码子下游的 AUG 密码子 (85-87) 内部起始而表达的,对应于大多数 ARFPs 的 C 末端部分。Core+1/S 是一种高度碱性的多肽,其功能仍不清楚。在这项工作中,未标记的重组 Core+1/S 从大肠杆菌中在天然条件下表达和纯化,并显示与 HCV 阳性患者的血清发生反应。随后,我们对 Core+1/S 进行了生化和生物物理特性分析。使用凝胶过滤层析、动态光散射、荧光、CD 和 NMR 研究了 Core+1/S 的构象和寡聚状态。与基于序列的无序预测一致,Core+1/S 在体外缺乏显著的二级结构,这可能与识别不同的分子伴侣和/或 Core+1/S 的组装有关。本研究是对 HCV ARFP/Core+1 蛋白的首次结构特征描述,并且提供了证据表明 ARFP/Core+1/S 在天然条件下高度无序,具有自我聚集的趋势。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验