快速分离蓝氏贾第鞭毛虫滋养体作为异黄酮芒柄花素的抗菌作用机制。
Rapid detachment of Giardia lamblia trophozoites as a mechanism of antimicrobial action of the isoflavone formononetin.
机构信息
Department of Pathology, University of California at San Diego, 214 Dickinson Street, San Diego, CA 92103-8416, USA.
出版信息
J Antimicrob Chemother. 2010 Mar;65(3):531-4. doi: 10.1093/jac/dkp501. Epub 2010 Jan 12.
Abstract
OBJECTIVES
Attachment to the small intestinal mucosa is crucial for initiating and maintaining Giardia infection. We tested the effect of isoflavones on Giardia attachment.
METHODS
We evaluated the effect of formononetin on trophozoite attachment to glass, to intestinal epithelial cell layers in vitro and to murine small intestinal explants, and on the intestinal load in mice.
RESULTS
We found that the isoflavone formononetin inhibits both attachment and flagellar motility within minutes and reduces the trophozoite load of Giardia in mice within 1.5 h after treatment.
CONCLUSIONS
The antigiardial activity of formononetin is at least partially due to its capacity to rapidly detach trophozoites.
摘要
目的
与小肠黏膜的附着对于启动和维持贾第虫感染至关重要。我们测试了异黄酮对贾第虫附着的影响。
方法
我们评估了芒柄花素对滋养体附着到玻璃、体外肠上皮细胞层和小鼠小肠外植体的影响，以及对小鼠肠道负荷的影响。
结果
我们发现异黄酮芒柄花素在数分钟内抑制附着和鞭毛运动，并在治疗后 1.5 小时内降低小鼠体内贾第虫的滋养体负荷。
结论
芒柄花素的抗贾第虫活性至少部分归因于其快速分离滋养体的能力。
相似文献
1
Rapid detachment of Giardia lamblia trophozoites as a mechanism of antimicrobial action of the isoflavone formononetin.
J Antimicrob Chemother. 2010 Mar;65(3):531-4. doi: 10.1093/jac/dkp501. Epub 2010 Jan 12.
2
Characterization of a Giardia lamblia WB C6 clone resistant to the isoflavone formononetin.
Microbiology (Reading). 2007 Dec;153(Pt 12):4150-4158. doi: 10.1099/mic.0.2007/010041-0.
3
Antigiardial activity of isoflavones from Dalbergia frutescens bark.
J Nat Prod. 2000 Oct;63(10):1414-6. doi: 10.1021/np000010d.
4
and effects of green-synthesized silver nanoparticles against infection.
Arch Razi Inst. 2024 Feb 1;79(1):218-225. doi: 10.32592/ARI.2024.79.1.218. eCollection 2024 Feb.
5
Giardia lamblia: evaluation of the in vitro effects of nocodazole and colchicine on trophozoites.
Exp Parasitol. 2005 May;110(1):62-72. doi: 10.1016/j.exppara.2005.01.007.
6
Activity of metronidazole, azithromycin and three benzimidazoles on Giardia lamblia growth and attachment to a human intestinal cell line.
Aliment Pharmacol Ther. 1994 Apr;8(2):187-92. doi: 10.1111/j.1365-2036.1994.tb00277.x.
7
Inhibitors of methionyl-tRNA synthetase have potent activity against Giardia intestinalis trophozoites.
Antimicrob Agents Chemother. 2015 Nov;59(11):7128-31. doi: 10.1128/AAC.01573-15. Epub 2015 Aug 31.
8
Synergic effect of metronidazole and pyrantel pamoate on Giardia lamblia.
Parasitol Int. 2011 Jan;60(1):54-8. doi: 10.1016/j.parint.2010.10.003. Epub 2010 Oct 12.
9
Activity of Thioallyl Compounds From Garlic Against Trophozoites and in Experimental Giardiasis.
Front Cell Infect Microbiol. 2018 Oct 15;8:353. doi: 10.3389/fcimb.2018.00353. eCollection 2018.
10
Giardia lamblia: the effects of extracts and fractions from Mentha x piperita Lin. (Lamiaceae) on trophozoites.
Exp Parasitol. 2007 Jan;115(1):25-31. doi: 10.1016/j.exppara.2006.05.001. Epub 2006 Jul 14.
引用本文的文献
1
Establishment of an in vitro co-infection model of Cryptosporidium parvum and Giardia duodenalis.
Parasit Vectors. 2025 Jul 12;18(1):281. doi: 10.1186/s13071-025-06926-5.
2
Formononetin exerts synergistic action with artesunate against multi-drug-resistant P. falciparum arresting ring-to-schizont transition by inducing reactive oxygen species.
Arch Microbiol. 2025 Apr 22;207(6):128. doi: 10.1007/s00203-025-04321-3.
3
Leishmanicidal and immunomodulatory activities of the formononetin (a natural isoflavone) against Leishmania tropica.
BMC Res Notes. 2023 Jun 26;16(1):120. doi: 10.1186/s13104-023-06403-1.
4
High-fat diet increases the severity of Giardia infection in association with low-grade inflammation and gut microbiota dysbiosis.
Sci Rep. 2021 Sep 22;11(1):18842. doi: 10.1038/s41598-021-98262-8.
5
activity of some natural honeys against and trophozoites.
Saudi J Biol Sci. 2019 Feb;26(2):238-243. doi: 10.1016/j.sjbs.2017.06.004. Epub 2017 Jun 6.
6
Identification and Validation of Small-Gatekeeper Kinases as Drug Targets in Giardia lamblia.
PLoS Negl Trop Dis. 2016 Nov 2;10(11):e0005107. doi: 10.1371/journal.pntd.0005107. eCollection 2016 Nov.
7
Drug Development Against the Major Diarrhea-Causing Parasites of the Small Intestine, Cryptosporidium and Giardia.
Front Microbiol. 2015 Nov 19;6:1208. doi: 10.3389/fmicb.2015.01208. eCollection 2015.
8
Giardia duodenalis Surface Cysteine Proteases Induce Cleavage of the Intestinal Epithelial Cytoskeletal Protein Villin via Myosin Light Chain Kinase.
PLoS One. 2015 Sep 3;10(9):e0136102. doi: 10.1371/journal.pone.0136102. eCollection 2015.
9
Drug Resistance in the Microaerophilic Parasite .
Curr Trop Med Rep. 2015;2(3):128-135. doi: 10.1007/s40475-015-0051-1.
10
Modeling long-term host cell-Giardia lamblia interactions in an in vitro co-culture system.
PLoS One. 2013 Dec 3;8(12):e81104. doi: 10.1371/journal.pone.0081104. eCollection 2013.
本文引用的文献
1
Tyrosine kinase-independent inhibition by genistein on spermatogenic T-type calcium channels attenuates mouse sperm motility and acrosome reaction.
Cell Calcium. 2009 Feb;45(2):133-43. doi: 10.1016/j.ceca.2008.07.004. Epub 2008 Sep 11.
2
Giardia duodenalis: adhesion-deficient clones have reduced ability to establish infection in Mongolian gerbils.
Exp Parasitol. 2008 Jul;119(3):364-72. doi: 10.1016/j.exppara.2008.03.010. Epub 2008 Mar 29.
3
Release of metabolic enzymes by Giardia in response to interaction with intestinal epithelial cells.
Mol Biochem Parasitol. 2008 Jun;159(2):85-91. doi: 10.1016/j.molbiopara.2008.02.005. Epub 2008 Feb 15.
4
Characterization of a Giardia lamblia WB C6 clone resistant to the isoflavone formononetin.
Microbiology (Reading). 2007 Dec;153(Pt 12):4150-4158. doi: 10.1099/mic.0.2007/010041-0.
5
Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy?
J Steroid Biochem Mol Biol. 2005 Apr;94(5):499-518. doi: 10.1016/j.jsbmb.2004.12.038. Epub 2005 Mar 23.
6
Molecular architecture of the sperm flagella: molecules for motility and signaling.
Zoolog Sci. 2003 Sep;20(9):1043-56. doi: 10.2108/zsj.20.1043.
7
Antigiardial activity of isoflavones from Dalbergia frutescens bark.
J Nat Prod. 2000 Oct;63(10):1414-6. doi: 10.1021/np000010d.
8
Differential inhibition of multiple cAMP phosphodiesterase isozymes by isoflavones and tyrphostins.
Mol Pharmacol. 2000 Apr;57(4):738-45. doi: 10.1124/mol.57.4.738.
9
Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile.
Trans R Soc Trop Med Hyg. 1983;77(4):487-8. doi: 10.1016/0035-9203(83)90120-7.
10
Attachment of Giardia-a hydrodynamic model based on flagellar activity.
J Exp Biol. 1974 Feb;60(1):207-21. doi: 10.1242/jeb.60.1.207.
Zotero 插件