[丹参的功能基因组学IV——乙烯反应元件结合蛋白基因分析]

[Functional genomics of Salvia militiorrhiza IV--analysis of ethylene responsive element binding protein gene].

作者信息

Xu Bin, Huang Luqi, Cui Guanghong, Mao Ying, Zhang Hui

机构信息

Institute of Chinese Materia Medical, China Academy of Chinese Medicinal Sciences, Beijing, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2564-6.

PMID:20069892

Abstract

OBJECTIVE

To study the ethylene responsive element binding protein genes of Salvia miltiorrhiza through bioinformatics and characterization of its tissue expression in regenerated plantlets.

METHOD

The ethylene responsive element binding protein genes were obtained by cDNA microarray analyze. BLAST was used for alignment, ORF finder software was used to find open reading frame, Prosite database was used to analyze the protein. Semi-quantitative RT- PCR method was used to detect the gene expression level.

RESULT

One ethylene responsive element binding protein was obtained, named as SmERF. SmERF had an open reading frame of 699 bp with 5'-URT 87 bp and 3'-URT 166 bp. The putative protein SmERF contains a highly conserved ERF/AP2 domain. Semiquantitative RT- PCR illustrated that SmERF was expressed in all tissues such as root, stem and leaf in regenerated shoots, while the expression level was higher in root than in stem and leaf.

CONCLUSION

It was the first time to obtain ERF gene in S. miltiorrhiza and set a good foundation for its further functional study.

摘要

目的

通过生物信息学方法研究丹参乙烯响应元件结合蛋白基因，并对其在再生植株中的组织表达特性进行分析。

方法

利用cDNA微阵列分析获得乙烯响应元件结合蛋白基因。采用BLAST进行序列比对，使用ORF finder软件查找开放阅读框，利用Prosite数据库分析蛋白质。采用半定量RT-PCR方法检测基因表达水平。

结果

获得一个乙烯响应元件结合蛋白，命名为SmERF。SmERF的开放阅读框为699 bp，5'-非编码区为87 bp，3'-非编码区为166 bp。推测的蛋白质SmERF含有一个高度保守的ERF/AP2结构域。半定量RT-PCR结果表明，SmERF在再生芽的根、茎、叶等所有组织中均有表达，且在根中的表达水平高于茎和叶。