Liu Jin-Ge, Zhang Zhen, Qin Qiu-Lin, Peng Ri-He, Xiong Ai-Sheng, Chen Jian-Min, Xu Fang, Zhu Hong, Yao Quan-Hong
Department of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, People's Republic of China.
Biotechnol Lett. 2007 Jan;29(1):165-73. doi: 10.1007/s10529-006-9214-4. Epub 2006 Nov 29.
A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L. by a yeast one-hybrid method. Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a conserved ethylene-responsive element binding protein (EREBP)/AP2 domain of 59 amino acids and a potential nuclear localization sequence. RCBF2 showed a high level of homology with other CBF family members only in AP2 domain. Phylogenetic analysis showed that RCBF2 might be different from other eight DRE-binding proteins on evolutionary relationship. The semi-quantitative RT-PCR (s-Q RT-PCR) analysis indicated the expression of RCBF2 gene was induced by cold, dehydration and high-salinity, but not by abscisic acid, and the transcription of RCBF2 gene accumulated primarily in rice immature seeds, growing point and shoots.
通过酵母单杂交方法从水稻中分离出一种与C-重复序列/脱水应答元件（C-repeat/DRE）相互作用的转录因子RCBF2。对推导的RCBF2氨基酸序列分析表明，RCBF2含有一个由59个氨基酸组成的保守乙烯应答元件结合蛋白（EREBP）/AP2结构域以及一个潜在的核定位序列。RCBF2仅在AP2结构域与其他CBF家族成员表现出高度同源性。系统发育分析表明，在进化关系上RCBF2可能与其他8种DRE结合蛋白不同。半定量RT-PCR（s-Q RT-PCR）分析表明，RCBF2基因的表达受低温、脱水和高盐诱导，但不受脱落酸诱导，且RCBF2基因转录主要在水稻未成熟种子、生长点和芽中积累。
